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| Status |
Public on Apr 10, 2013 |
| Title |
Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts [CLIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
MicroRNAs (miRNAs) are critical to proliferation, differentiation, and development. Here, we characterize gene expression in murine Dicer-null adult mesenchymal stem cell lines, a fibroblast cell type. Loss of Dicer leads to de-repression of let-7 targets at levels that exceed 10-100 fold with increases in transcription. Direct and indirect targets of this miRNA belong to a mid-gestation embryonic program that encompasses known oncofetal genes as well as oncogenes not previously associated with an embryonic state. Surprisingly, this mid-gestation program represents a distinct period that occurs between the pluripotent state of the inner cell mass at embryonic day 3.5 and the induction of let-7, upon differentiation, at embryonic day 10.5. Within this mid-gestation program, we characterize the let-7 target Nr6a1, an embryonic transcriptional repressor that regulates gene expression in adult fibroblasts following miRNA loss. In total, let-7 is required for the continual suppression of embryonic gene expression in adult cells, a mechanism that may underlie its tumor suppressive function.
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| Overall design |
Adult mesenchymal stem cells (immortalized monoclonal lines of murine MSCs) with Dicer (WT: Dicer f/f), were infected with dox-inducible Flag-HA-hAgo2 or untagged hAgo2 and then UV cross-linked. An immunoprecipitation was carried out with anti-Flag and anti-HA antibodies, and associated RNAs were sequenced.
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| Contributor(s) |
Gurtan AM, Ravi A, Rahl PB, Bosson AD, JnBaptiste CK, Bhutkar A, Whittaker CA, Young RA, Sharp PA |
| Citation(s) |
23630078 |
| Submission date |
Apr 05, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
A Bhutkar |
| Organization name |
MIT
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| Street address |
77 Massachusetts Avenue
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE44163 |
Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts |
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| Relations |
| BioProject |
PRJNA196441 |
| SRA |
SRP020560 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45828_MSC_top30miRNA_sites_expressed_above_0.1FPKM_CLIPannotated.bed.txt.gz |
11.2 Mb |
(ftp)(http) |
TXT |
| GSE45828_RAW.tar |
133.8 Mb |
(http)(custom) |
TAR (of BW, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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