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Series GSE45719 Query DataSets for GSE45719
Status Public on Jan 10, 2014
Title Single-cell RNA-Seq reveals dynamic, random monoallelic gene expression in mammalian cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In the diploid genome, genes come in two copies, which can have different DNA sequence and where one is maternal and one is paternal. In a particular cell, a gene could potentially be expressed from both copies (biallelic expression) or only one (monoallelic). We performed RNA-Sequencing on individual cells, from zygote to the cells of the late blastocyst, and also individual cells from the adult liver. Using first generation crosses between two distantly related mouse strains, CAST/Ei and C57BL/6, we determined the expression separately from the maternal and paternal alleles. We found that half of the genes were expressed by only one allele, randomly so that some cells would express the paternal allele, some the maternal and a few cell both alleles. We also observed the spread of the progressive inactivation of the paternal X chromosome.
Overall design First generation mouse strain crosses were used to study monoallelic expression on the single cell level
Contributor(s) Deng Q, Ramsköld D, Reinius B, Sandberg R
Citation(s) 24408435
Submission date Apr 02, 2013
Last update date May 15, 2019
Contact name Daniel Ramsköld
Organization name Karolinska Institutet
Lab Rickard Sandberg's group ( (2008-2014, 2018-); Rheumatology unit (2014-2018)
Street address Nobels väg 3
City Stockholm
ZIP/Postal code 17177
Country Sweden
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (317)
GSM1112490 16cell_1-10
GSM1112491 16cell_1-11
GSM1112492 16cell_1-12
BioProject PRJNA195938
SRA SRP020490

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45719_RAW.tar 97.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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