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Series GSE45593 Query DataSets for GSE45593
Status Public on Apr 01, 2013
Organism Homo sapiens
Experiment type Third-party reanalysis
Expression profiling by array
Summary Immunosuppression is needed in HLA identical sibling renal transplantation. We conducted a tolerance trial in this patient cohort using Alemtuzumab induction, donor hematopoietic stem cells, tacrolimus/mycophenolate immunosuppression converted to sirolimus, planning complete drug withdrawal by 24 months post-transplantation. After an additional 12 months with no immunosuppression, normal biopsies and renal function, recipients were considered tolerant. Twenty recipients were enrolled. Of the first 10 (>36 months post-transplantation), 5 had immunosuppression successfully withdrawn for 16-36 months (tolerant), 2 had disease recurrence and 3 had subclinical rejection in protocol biopsies (non-tolerant). Microchimerism disappeared after 1 year, and CD4+CD25highCD127-FOXP3+ T cells and CD19+IgD/M+CD27- B cells increased to 5 years post-transplantation in both groups, whereas immune/inflammatory gene expression pathways in the peripheral blood and urine were differentially downregulated in tolerant compared to non-tolerant recipients. Therefore, in this HLA identical renal transplant tolerance trial, absent chimerism, Treg and Breg immunophenotypes were indistinguishable between tolerant and non-tolerant recipients, but global genomic changes indicating immunomodulation were observed only in tolerant recipients.
Overall design A total of 46 PBMC samples representing blood draws from four time points in the first 9 recipients were processed for microarray analysis (The Scripps Research Institute, La Jolla, CA). The analyzed time points were: immediately pre-operatively in the absence of immunosuppression (n=9); post-operatively at 1 year (n=8, range 11-13 months); at 2 years (n=12, range 18-25 months); >3 years (n=17, range 32-48 months). (At year 2 and at > 3 years, repeated samples were obtained from individual subjects, and at one year, one subject had a technically unsatisfactory sample.) To discount the effects of immunosuppression on gene expression, microarray data were included on whole blood from 18 healthy human subjects (controls: GSE40586; NCBI Gene Expression Omnibus [GEO] repository).
Contributor(s) Kurian SM
Citation(s) 23787913
Submission date Mar 28, 2013
Last update date Jul 26, 2018
Contact name Sunil Kurian
Phone 858-784-7759
Organization name The Scripps Research Institute
Department Molecular and Experimental Medicine
Street address 10550 N Torrey Pines Road
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (2)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (46)
GSM1110225 PBMC_1_0
GSM1110226 PBMC_1_12
GSM1110227 PBMC_1_24
Reanalysis of GSM997333
Reanalysis of GSM997343
Reanalysis of GSM997345
Reanalysis of GSM997347
Reanalysis of GSM997358
Reanalysis of GSM997361
Reanalysis of GSM997363
Reanalysis of GSM997342
Reanalysis of GSM997351
Reanalysis of GSM997341
Reanalysis of GSM997369
Reanalysis of GSM997370
Reanalysis of GSM997368
Reanalysis of GSM997337
Reanalysis of GSM997360
Reanalysis of GSM997366
Reanalysis of GSM997338
Reanalysis of GSM997334
BioProject PRJNA194628

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45593_RAW.tar 185.6 Mb (http)(custom) TAR (of CEL)
GSE45593_log2_RMA_all_samples.txt.gz 7.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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