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Series GSE45504 Query DataSets for GSE45504
Status Public on Jun 26, 2013
Title Muscleblind-like proteins regulate embryonic stem cell-specific alternative splicing and reprogramming II
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Previous investigations of the core gene regulatory circuitry that controls embryonic stem cell (ESC) pluripotency have largely focused on the roles of transcription, chromatin and non- coding RNA regulators. Alternative splicing (AS) represents a widely acting mode of gene regulation, yet its role in the regulation of ESC pluripotency and differentiation is poorly understood. Here, we identify the Muscleblind-like RNA binding proteins, MBNL1 and MBNL2, as conserved and direct negative regulators of a large program of AS events that are differentially regulated between ESCs and other cell types. Knockdown of MBNL proteins in differentiated cells causes switching to an ESC-like AS pattern for at least half of these AS events. Among the events is an ESC-specific AS switch in the forkhead family transcription factor FOXP1 that controls pluripotency. Consistent with a central and negative regulatory role for MBNL proteins in pluripotency, their knockdown significantly enhances the expression of key pluripotency genes and the formation of induced pluripotent stem cells (iPSCs) during somatic cell reprogramming.
Overall design mRNA profiles of various embryonic stem cells, tissues and cell lines from human and mouse using high-throughput sequencing data and the role of MBNL proteins in regulation of ESC-differential alternative splicing
Contributor(s) Han H, Irimia M, Blencowe BJ
Citation(s) 23739326
Submission date Mar 26, 2013
Last update date May 15, 2019
Contact name Manuel Irimia
Phone +34933160212
Organization name Centre for Genomic Regulation
Department Systems Biology Unit
Street address 88 Dr. Aiguader
City Barcelona
State/province Barcelona
ZIP/Postal code 08003
Country Spain
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (5)
GSM1105743 293T nt_control
GSM1105744 293T siMBNL1+2
GSM1105745 HeLa nt_control
This SubSeries is part of SuperSeries:
GSE45505 Muscleblind-like proteins regulate embryonic stem cell-specific alternative splicing and reprogramming
SRA SRP019989
BioProject PRJNA195386

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45504_RAW.tar 1.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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