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Series GSE45385 Query DataSets for GSE45385
Status Public on Mar 21, 2016
Title Toll-like Receptor 6 and Connective Tissue Growth Factor are significantly upregulated in mitomycin-C treated urothelial carcinoma cells under hydrostatic pressure stimulation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Urothelial carcinoma (UC) is the most common histologic subtype of bladder cancer. The administration of mitomycin C (MMC) into bladder after transurethral resection of the bladder tumor (TURBT) is a common treatment strategy for preventing recurrence after surgery. We previously applied hydrostatic pressure combined with MMC in UC cells and found that hydrostatic pressure synergistically enhanced MMC-induced UC cell apoptosis via the Fas/FasL pathways. To understand the alteration of gene expressions in UC cells caused by hydrostatic pressure and MMC, oligonucleotide microarray was used to explore all of the differentially expressed genes. After bioinformatics analysis and gene annotation, Toll-like receptor 6 (TLR6) and connective tissue growth factor (CTGF) showed significant up-regulation among altered genes, and their expressions with each treatment of UC cells were validated by quantitative real-time PCR (qPCR). We conclude that under treatment with MMC and hydrostatic pressure, UC cells showed increasing apoptosis via extrinsic pathways through upregulation of TLR6 and CTGF.
Overall design BFTC905 cells, a UC cell line, were cultured in the presence of 10 μg/ml of MMC for 2.5 hours. The MMC-treated cells were then incubated in a well-designed hydrostatic bioreactor for 10 kPa pressure treatment in duplicate or were cultured as usual to be used as control sets.
Contributor(s) Chen S, Chung C, Cheng Y, Huang C, Chen W, Ruaan R, Li C, Tsao C, Hu W, Chien C
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Submission date Mar 21, 2013
Last update date Nov 27, 2018
Contact name Chih-Cheng Chien
Organization name Sijhih Cathay General Hospital
Street address No 2, Ln 59, Jian-Cheng Rd, Sijhih Dist
City New Taipei
ZIP/Postal code 22174
Country Taiwan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (4)
GSM1103453 BFTC905_M10_0kPa_rep1.
GSM1103454 BFTC905_M10_10kPa_rep1.
GSM1103455 BFTC905_M10_0kPa_rep2.
BioProject PRJNA193606

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Supplementary file Size Download File type/resource
GSE45385_RAW.tar 54.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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