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Series GSE45378 Query DataSets for GSE45378
Status Public on Apr 21, 2013
Title HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Analysis of histone H3 turnover in wild-type and mutant fission yeast cells by using MNase-ChIP-chip
 
Overall design Exponentially growing cultures of fission yeast cells expressing an ectopic copy of FLAG tagged histone H3 under the control of inv1 promoter were synchronized and expression of H3-FLAG was induced by changing the carbon source of the medium to Sucrose. Cells were crosslinked with 1% Formaldehyde and chromatin was fragmented into mononucleosomes by using micrococcal nuclease (MNase). Chromatin immunoprecipitated DNA recovered with anti-FLAG antibody from wild-type and mutant fission yeast cells and whole cell extracts DNA were amplified by random priming and respectively labeled with Cy5 and Cy3. Labeled DNA samples were mixed and hybridized onto 44k pombe Agilent oligo arrays. Data were processed using Agilent scanner and Feature Extraction software.
 
Contributor(s) Aygün O, Mehta S, Grewal SI
Citation(s) 23604080
Submission date Mar 21, 2013
Last update date Jul 21, 2013
Contact name Shiv Grewal
Phone 2407607553
Organization name NCI
Department LBMB
Lab Shiv Grewal
Street address NCI bldg 37 Rm 6068 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (2)
GPL11244 NCI_GREWAL_Spombe_C10_v1
GPL16854 Agilent-039363 Schizosaccharomyces pombe 44K oligo array Ozan_C10S
Samples (13)
GSM1103380 H3 turnover in Wild-type cells
GSM1103381 H3 turnover in clr4 deletion mutant
GSM1103382 H3 turnover in dcr1 deletion mutant
Relations
BioProject PRJNA193687

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45378_RAW.tar 58.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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