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| Status |
Public on May 27, 2013 |
| Title |
Genome-wide mapping of Runx1-bound sites in early B-cell progenitors |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The t(12;21) chromosomal translocation, targeting the gene encoding the RUNX1 transcription factor, is observed in 25% of pediatric acute lymphoblastic leukemia (ALL) and is an initiating event in the disease. To elucidate the mechanism by which RUNX1 disruption initiates leukemogenesis, we investigated its normal role in murine B-cell development. Gene expression analysis and genome-wide Runx1-occupancy studies support the hypothesis that Runx1 reinforces the transcription factor network in B-cell progenitors governing early B-cell survival and development .
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| Overall design |
ChIP-seq experiments were performed in the proB-cell line BMiFLT3(15-3), stably transduced with the transcription factor Runx1, to identify Runx1-bound sites in early B-cell progenitors.
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| Contributor(s) |
Niebuhr B, Kriebitzsch N, Alawi M, Stocking C |
| Citation(s) |
23704093 |
| Submission date |
Mar 21, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Neele Margarete Kriebitzsch |
| E-mail(s) |
neele.kriebitzsch@hpi.uni-hamburg.de
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| Organization name |
Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology
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| Lab |
Molecular Pathology
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| Street address |
Martinistraße 52
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| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE45425 |
Runx1 targets in early B-cell progenitors |
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| Relations |
| SRA |
SRP019917 |
| BioProject |
PRJNA193697 |
