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Series GSE45373 Query DataSets for GSE45373
Status Public on Mar 22, 2013
Title Cell-autonomous function of Runx1 transcriptionally regulates megakaryocytic maturation in mice (expression)
Organism Mus musculus
Experiment type Expression profiling by array
Summary RUNX1 transcription factor (TF) is a key regulator of megakaryocytic development and when mutated is associated with familial platelet disorder and predisposition to acute myeloid leukemia (FPD-AML). We used mice lacking Runx1 specifically in megakaryocytes (MKs) to characterize the Runx1-mediated transcriptional program during advanced stages of MK differentiation. Gene expression and chromatin-immunoprecipitation-sequencing (ChIP-seq) of Runx1 and p300 identified functional Runx1-bound MK enhancers. Runx1/p300 co-bound regions showed significant enrichment in genes important for MK and platelet homeostasis. Runx1-bound regions were highly enriched in RUNX and ETS motifs and to a lesser extent in GATA motif. The data provides the first example of genome-wide Runx1/p300 occupancy in maturating FL-MK, unravels the Runx1-regulated program controlling MK maturation in vivo and identifies its bona fide regulated genes. It advances our understanding of the molecular events that upon mutations in RUNX1 lead to the predisposition to familial platelet disorders and FPD-AML.
Overall design Gene expression profiles of mature megakaryocytes taken from fetal livers of megakaryocyte-specific Runx1 knockout mice, using Runx1F/F/Pf4-Cre mice versus control (WT) mice.
Contributor(s) Pencovich N, Jaschek R
Citation(s) 23717578
Submission date Mar 21, 2013
Last update date Mar 04, 2019
Contact name Ram Jaschek
Organization name Weizmann Institute of Science
Street address P.O Box 26
City Rechovot
ZIP/Postal code 71600
Country Israel
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (6)
GSM1103357 InVivo_WT_1
GSM1103358 InVivo_KO_1
GSM1103359 InVivo_WT_2
This SubSeries is part of SuperSeries:
GSE45374 Cell-autonomous function of Runx1 transcriptionally regulates megakaryocytic maturation in mice
BioProject PRJNA193575

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45373_RAW.tar 23.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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