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Series GSE45350 Query DataSets for GSE45350
Status Public on Mar 21, 2013
Title Genome-wide screening of the expression of long noncoding RNA and coding RNA expression in esophageal sqaumous cell cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary In order to understand the role of long noncoding RNAs (lncRNAs) and their interaction with coding RNAs in esophageal sqaumous cell cancer (ESCC), we performed genome-wide screening of the expression of lncRNAs and coding RNAs from primary ESCC tissue and adjacent normal tissue using Agilent SurePrint G3 Human GE 8x60K Microarray. By comparing ESCC tissues and matched normal tissues, differentially expressed lncRNAs and coding RNAs were identified and confirmed with PCR and other independent studies. We further identified a subset of co-located and co-expressed lncRNAs and coding RNAs using bioinformatic tools and the analysis suggested that a subset of lncRNAs may influence nearby genes involved in the genesis of ESCC.
Overall design Four pairs of ESCC primary tumors and adjacent normal tissues were used for genome-scale microarray experiments, which included long noncoding RNAs and coding RNAs. Selected lncRNAs expressed in the experiment were validated on independent matched-pair samples with PCR method.
Contributor(s) Cao W, Wu W, Shi F
Citation(s) 25297587
Submission date Mar 20, 2013
Last update date Nov 27, 2018
Contact name Wei Wu
Organization name 1.Zhengzhou Central Hospital, Affiliated to Zhengzhou University, China; 2. University of Calgary
Street address 3330 Hospital Dr. NW
City Calgary
ZIP/Postal code T2N 4N1
Country Canada
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (8)
GSM1102826 Normal esophageal tissue from patient 1 with ESCC
GSM1102827 Primary ESCC tissue from patient 1 with ESCC
GSM1102828 Normal esophageal tissue from patient 2 with ESCC
BioProject PRJNA193519

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45350_RAW.tar 30.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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