 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 16, 2013 |
| Title |
Expression data from HT-29, a human colonic epithelial cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene(s) in epithelial cells can be upregulated or downregulated by different stimuli in a tissue-specific manner. We used genome-wide microarray analysis to profile the expression of genes in HT-29 cells that are upregulated after stimulation with sodium butyrate (NaB) and subsequently downregulated by the addition of ciprofloxacin antibiotic. The specific aim was to evaluate genes that are associated with mucosal immunity.
|
| |
|
| Overall design |
HT-29 cells were stimulated with sodium butyrate (NaB) (n=3), NaB and ciprofloxacin (n=3) or culture media (unstimulated control, n=3) for 24 hours. RNA extracted from these cells were hybridized on Affymetrix microarrays.
|
| |
|
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 15, 2013 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Protim Sarker |
| E-mail(s) |
protim@icddrb.org
|
| Phone |
+8801772683211
|
| Organization name |
icddr,b
|
| Street address |
Mohakhali
|
| City |
Dhaka |
| ZIP/Postal code |
1212 |
| Country |
Bangladesh |
| |
|
| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
|
| Samples (9)
|
| GSM1099310 |
HT-29, unstimulated, replicate #1 |
| GSM1099311 |
HT-29, stimulated with NaB, replicate #1 |
| GSM1099312 |
HT-29, stimulated with NaB and ciprofloxacin, replicate #1 |
| GSM1099313 |
HT-29, unstimulated, replicate #2 |
| GSM1099314 |
HT-29, stimulated with NaB, replicate #2 |
| GSM1099315 |
HT-29, stimulated with NaB and ciprofloxacin, replicate #2 |
| GSM1099316 |
HT-29, unstimulated, replicate #3 |
| GSM1099317 |
HT-29, stimulated with NaB, replicate #3 |
| GSM1099318 |
HT-29, stimulated with NaB and ciprofloxacin, replicate #3 |
|
| Relations |
| BioProject |
PRJNA193228 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45220_RAW.tar |
40.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...