GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE45220 Query DataSets for GSE45220
Status Public on Mar 16, 2013
Title Expression data from HT-29, a human colonic epithelial cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Gene(s) in epithelial cells can be upregulated or downregulated by different stimuli in a tissue-specific manner. We used genome-wide microarray analysis to profile the expression of genes in HT-29 cells that are upregulated after stimulation with sodium butyrate (NaB) and subsequently downregulated by the addition of ciprofloxacin antibiotic. The specific aim was to evaluate genes that are associated with mucosal immunity.
Overall design HT-29 cells were stimulated with sodium butyrate (NaB) (n=3), NaB and ciprofloxacin (n=3) or culture media (unstimulated control, n=3) for 24 hours. RNA extracted from these cells were hybridized on Affymetrix microarrays.
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 15, 2013
Last update date Nov 08, 2016
Contact name Protim Sarker
Phone +8801772683211
Organization name icddr,b
Street address Mohakhali
City Dhaka
ZIP/Postal code 1212
Country Bangladesh
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (9)
GSM1099310 HT-29, unstimulated, replicate #1
GSM1099311 HT-29, stimulated with NaB, replicate #1
GSM1099312 HT-29, stimulated with NaB and ciprofloxacin, replicate #1
BioProject PRJNA193228

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45220_RAW.tar 40.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap