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Series GSE45185 Query DataSets for GSE45185
Status Public on Jan 22, 2015
Title Genetic diversity of propagating/stem cells in glioblastoma multiforme (GBM)
Organism Homo sapiens
Experiment type SNP genotyping by SNP array
Genome variation profiling by SNP array
Summary Glioblastoma multiforme (GBM) is a lethal malignancy whose clinical intransigence has been linked to extensive intra-clonal genetic and phenotypic diversity and therapeutic resistance of cancer stem cells (CSCs). This interpretation embodies an implicit assumption that CSCs are themselves genetically diverse. To test this, we screened neurosphere cultures by SNP arrays to identify copy number alterations (CNA) (minimum of three) that could be visualised in single cells by multi-colour FISH. Interrogation of neurosphere-derived cells (from four patients) and cells derived from secondary transplants of these same cells in Nod/Scid mice allowed us to infer clonal phylogenic architecture and the likely derivation of functional CSCs. This proof-of-principle experiment revealed that more than one sub-clone (but not all) in each GBM had functionally defined, genetically distinct stem cells.
Overall design Diagnostic and germ line DNA from 12 patients with GBM was investigated for LOH/CNA using Affymetrix SNP 6.0 arrays. Neurosphere cultures were established from these primary tumors, and intracranially injected into Nod/Scid mice.
Clonal architecture of primary neurospheres and secondary transplants was investigated by multicolour FISH, guided by the LOH/CNA analysis of the primary GBM tumors.
Contributor(s) Piccirillo S, van Delft FW, Potter N, Colman SM, Watts C, Kearney L, Greaves M
Citation(s) 25533637
Submission date Mar 14, 2013
Last update date Nov 27, 2018
Contact name Frederik van Delft
Organization name ICR
Department Haemato-oncology
Street address 15 Cotswold Road
City Belmont
ZIP/Postal code SM2 5NG
Country United Kingdom
Platforms (1)
GPL6801 [GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array
Samples (24)
GSM1098737 GL1
GSM1098738 GBM1
GSM1098739 GL2
BioProject PRJNA193175

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45185_RAW.tar 987.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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