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| Status |
Public on Aug 20, 2013 |
| Title |
Interrogating translating RNAs and cell-type specific gene expression using Cre-activated translating ribosome affinity purification |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We characterize a Cre-activated TRAP allele (Rosa26fsTRAP) to show that endothelium-specific activation of Rosa26fsTRAP identifies endothelial cell enriched transcripts, and that cardiomyocyte-restricted TRAP is a useful means to identify genes that are differentially expressed in cardiomyocytes in a disease model. We also show that TRAP is an effective means to study translational regulation, and we several nuclear-encoded mitochondrial genes are under strong translational control. These RNA-seq data show that the TRAP strategy and the Rosa26fsTRAP allele will be useful tools to probe cell type specific transcriptomes, and to study translational regulation.
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| Overall design |
Study TRAP RNA and total RNA in two cases: heart specific tissues, Banding or Sham, using RNA-seq technology.
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| Contributor(s) |
Zhou P, Zhang Y, Gu F, Ma Q, Day DS, He A, Zhou1 B, Li J, Stevens S, Romo D, Park PJ, Pu WT |
| Citation(s) |
24003143 |
| Submission date |
Mar 13, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Fei Gu |
| E-mail(s) |
alickgf@hotmail.com
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| Organization name |
Childrens hospital boston, Harvard Medical School
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| Department |
Cardiology
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| Lab |
William Pu
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| Street address |
320 Longwood Ave.
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (53)
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| Relations |
| SRA |
SRP019266 |
| BioProject |
PRJNA193343 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45152_RAW.tar |
1.1 Gb |
(http)(custom) |
TAR (of BEDGRAPH, BIGWIG) |
| GSE45152_RPKM.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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