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Series GSE45152 Query DataSets for GSE45152
Status Public on Aug 20, 2013
Title Interrogating translating RNAs and cell-type specific gene expression using Cre-activated translating ribosome affinity purification
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We characterize a Cre-activated TRAP allele (Rosa26fsTRAP) to show that endothelium-specific activation of Rosa26fsTRAP identifies endothelial cell enriched transcripts, and that cardiomyocyte-restricted TRAP is a useful means to identify genes that are differentially expressed in cardiomyocytes in a disease model. We also show that TRAP is an effective means to study translational regulation, and we several nuclear-encoded mitochondrial genes are under strong translational control. These RNA-seq data show that the TRAP strategy and the Rosa26fsTRAP allele will be useful tools to probe cell type specific transcriptomes, and to study translational regulation.
Overall design Study TRAP RNA and total RNA in two cases: heart specific tissues, Banding or Sham, using RNA-seq technology.
Contributor(s) Zhou P, Zhang Y, Gu F, Ma Q, Day DS, He A, Zhou1 B, Li J, Stevens S, Romo D, Park PJ, Pu WT
Citation(s) 24003143
Submission date Mar 13, 2013
Last update date May 15, 2019
Contact name Fei Gu
Organization name Childrens hospital boston, Harvard Medical School
Department Cardiology
Lab William Pu
Street address 320 Longwood Ave.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (53)
GSM1098097 TNT_Band_input_rep1
GSM1098098 TNT_Band_TRAP_rep1
GSM1098099 TNT_Sham_input_rep1
SRA SRP019266
BioProject PRJNA193343

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45152_RAW.tar 1.1 Gb (http)(custom) TAR (of BEDGRAPH, BIGWIG)
GSE45152_RPKM.txt.gz 1.6 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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