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Series GSE45124 Query DataSets for GSE45124
Status Public on Jan 01, 2014
Title Genome wide mapping of PSF binding sites in prostate cancer cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Prostate cancer is the most common cancer in men and AR downstream signalings promote prostate cancer cell proliferation. We identified androgen-regulated long non-coding RNA, CTBP1-AS, located in the antisese region of CTBP1 gene. CTBP1-AS activate AR signaling by epigenetically repress AR-associated cofactors such as CTBP1 by interactign with RNA-binding protein PSF and recruiting HDAC complex to the target promoters. In order investigated the PSF target genes, we performed ChIP-seq analysis of PSF binding sites in prostate cancer cell line, LNCaP cells. We identified androgen dependent PSF binding regions in prostate cancer cell genome. We observed PSF bindings around the promoters of androgen repressed genes such as CTBP1, p53 and SMAD3.
Overall design ChIP-sequence analysis of PSF binding sites in prostate cancer cells
Contributor(s) Takayama K, Tsutsumi S, Inoue S, Aburatani H
Citation(s) 23644382
Submission date Mar 13, 2013
Last update date Jan 11, 2021
Contact name Ken-ichi Takayama
Organization name Tokyo Metropolitan Institute of Gerontology
Street address Sakaecho
City Itabashi-ku
ZIP/Postal code 173-0015
Country Japan
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (4)
GSM1097496 PSF_Vehicle_ChIP
GSM1097497 PSF_R1881_ChIP
GSM1097498 PSF_Vehicle_Input
BioProject PRJNA192937
SRA SRP019259

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45124_RAW.tar 37.3 Mb (http)(custom) TAR (of BAR)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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