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Series GSE44935 Query DataSets for GSE44935
Status Public on Mar 08, 2013
Title The Role of Tet1 During Somatic Cell Reprogramming
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Tet1 is a hydroxylase known for its role in the conversion of 5-methylcytosines (5mC) to 5-hydroxymethylcytosines (5hmC) involved in the possible active demethylation process and gene expression regulation. As somatic cell reprogramming involves the re-activation of pluripotency genes and the silencing of somatic ones, it remains unclear the role of Tet1 in the reprogramming process. Here, we performed hMeDIP-seq and RNA-seq during somatic cells reprogramming with Tet1 over expression to invest the effect of Tet1.
Overall design Examination of genome DNA hmC modifications in 2 conditions: individually overexpressed Tet1CD or Tet2CD during MEF reprogramming; Examination of mRNA levels in five different conditions: individually overexpressed DR or Tet1CD or Tet1CDmut or Tet2CD or Tet2CDmut, during MEF reprogrammig.
Contributor(s) Pei D, Xu G
Citation(s) 24162740
Submission date Mar 06, 2013
Last update date May 15, 2019
Contact name Lihui Lin
Phone 020-32015291
Organization name Guangzhou Institutes Of Biomedicine and Health Chinese Academy Of Science
Department Stem cell
Lab Pei Duanqing
Street address No.190, Kaiyuan Street
City Guangzhou
State/province Guangdong
ZIP/Postal code GD20
Country China
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (7)
GSM1094149 Tet1CD_hMeDIP-seq
GSM1094150 Tet2CD_hMeDIP-seq
GSM1094151 DR_RNAseq
BioProject PRJNA192583
SRA SRP018969

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Supplementary file Size Download File type/resource
GSE44935_RAW.tar 2.7 Mb (http)(custom) TAR (of BED, TXT)
GSE44935_Total_samples_expression_values.txt.gz 205.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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