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Series GSE44830 Query DataSets for GSE44830
Status Public on Mar 06, 2013
Title Treatment of AML patient samples with decitabine
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Genome wide DNA methylation profiling of AML patient samples treated with PBS or DAC. The Illumina Infinium 450 Human DNA methylation was used to examine the methylation profile of 8 patient samples and 2 cell lines.

Genome wide DNA methylation profiling of AML xenografts treated with either PBS control or with decitacine (DAC) alone, cytarabine (Ara-C) alone, DAC and Ara-C together (D+A), DAC followed by Ara-C (D/A) or with Ara-C followed by DAC (A/D).
 
Overall design DNA was extracted from patient bone marrow samples and xenograft bone marrow samples using Qiagen Allprep kit. Bisulphite converted DNA from all samples were hybridised to the Illumina Infinium 450 Human Methylation arrays and for each analysis the drug treated sample was compared to the corresponding PBS control sample.
 
Contributor(s) Leonard S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 04, 2013
Last update date Mar 22, 2019
Contact name sarah leonard
E-mail(s) sarleonard@gmail.com
Organization name University of Birmingham
Department School of cancer sciences
Street address Edgbaston
City Birmingham
ZIP/Postal code B15 2TT
Country United Kingdom
 
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (26)
GSM1092170 genomic DNA from AML patient 1 [AML-1 PBS treated]
GSM1092173 genomic DNA from AML patient 1 [AML-1 DAC treated]
GSM1092175 genomic DNA from AML patient 2 [AML-2 PBS treated]
Relations
BioProject PRJNA192434

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44830_RAW.tar 394.9 Mb (http)(custom) TAR (of IDAT)
GSE44830_matrix_signal_intensities.txt.gz 143.4 Mb (ftp)(http) TXT
GSE44830_readme.txt.gz 320 b (ftp)(http) TXT
Processed data included within Sample table
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