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Series GSE44775 Query DataSets for GSE44775
Status Public on May 15, 2013
Title Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The degradation of TNFα mRNA is critical for restricting TNFα synthesis and involves a constitutive decay element (CDE) in the 3’ untranslated region of the mRNA. We demonstrate that the active CDE is a stem-loop RNA motif that is recognized by the RNA-binding protein Roquin. Deep sequencing of Roquin-associated mRNAs identified CDE-containing mRNAs as the primary targets of Roquin on a transcriptome-wide scale.
Overall design Expression profiles of LPS treated Raw264.7 cells (input), after Roquin IP or control IP were generated by deep sequencing, in three biological replicates, using Illumina HiSeq 2000.
Contributor(s) Leppek K, Schott J, Reitter S, Poetz F, Hammond MC, Stoecklin G
Citation(s) 23663784
Submission date Mar 01, 2013
Last update date May 15, 2019
Contact name Georg Stoecklin
Organization name German Cancer Research Center
Street address Im Neuenheimer Feld 280
City Heidelberg
ZIP/Postal code 69120
Country Germany
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM1090971 Roquin_IP1
GSM1090972 Roquin_IP2
GSM1090973 Roquin_IP3
BioProject PRJNA191615
SRA SRP019223

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44775_RoquinIP_processed.txt.gz 262.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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