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Series GSE44744 Query DataSets for GSE44744
Status Public on Oct 17, 2013
Title Krüppel-like factor 3 KO and rescue expression data in murine embyronic fibroblasts
Organism Mus musculus
Experiment type Expression profiling by array
Summary Krüppel-like factor 3 (KLF3) is a transcriptional repressor that has roles in adipogenesis, B-cell maturation and erythropoiesis (for review see Pearson et al., 2012).
We profiled gene expression in murine embryonic fibroblasts (MEFs) where Klf3 had been knocked-out and where the same cell had been rescued with Klf3 using microarrays.
Overall design Klf3 KO murine embryonic fibroblast cell lines were produced from Klf3 KO mice. These cells were rescued with epitope tagged Klf3 (Klf3-V5) using retroviral delivery (murine stem cell virus). Stable clones were selected uner puromycin seletion and total RNA was taken for array
Contributor(s) Burdach J, Funnell AP, Artuz CM, Sin MK, Tan LY, Pearson RC, Crossley M
Citation(s) 24106088
Submission date Feb 28, 2013
Last update date Apr 18, 2017
Contact name Jon Burdach
Organization name University of New South Wales
Department BABS
Street address UNSW
City Sydney
State/province NSW
ZIP/Postal code 2052
Country Australia
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (4)
GSM1089967 MEF_Klf3-/-_rep1
GSM1089968 MEF_Klf3-/-_rep2
GSM1089969 MEF_Klf3-Rescue_rep1
BioProject PRJNA191576

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Supplementary file Size Download File type/resource
GSE44744_RAW.tar 17.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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