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Series GSE44741 Query DataSets for GSE44741
Status Public on Aug 27, 2013
Title Genome-Wide Binding of Ehf in mouse corneal epithelium
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The cornea, composed of epithelium, stroma and endothelium, protects the anterior compartment of the eye from damage and allows transmission of light into the eye. While well described morphologically, no studies have investigated the global gene expression changes in the cornea throughout the mouse’s life. We characterized the global gene expression profile of mouse cornea from early development through aging, and compared to gene expression in other epithelial tissue, to identify cornea enriched genes, pathways, and transcriptional regulators. We identified Ehf, an ets family transcription factor, as being highly selectively expressed in the corneal epithelium compared to the stroma, and highly expressed in cornea compared to other epithelial tissues. siRNA experiments and Ehf ChIP-Seq on mouse corneal epithelium confirm the role of this factor in promoting epithelial identity and cell differentiation, and suggest it carries out these functions through interactions with other cornea epithelial differentiation factors including Klf4.
Overall design Whole eye globes were dissected from wild type CB6 mice. Corneal epithelium was isolated by digestion in 50% EMEM/dispase II with 50 mM sorbitol for two hours at 37°C. ChIP was performed with an Ehf antibody, and was sequenced with an input control.
Contributor(s) Klein RH, Stephens D, Andersen B
Citation(s) 24142692
Submission date Feb 28, 2013
Last update date May 15, 2019
Contact name Bogi Andersen
Organization name University of California, Irvine
Department Medicine
Lab Andersen
Street address 839 Health Sciences Dr.
City Irvine
State/province CA
ZIP/Postal code 92697
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1089893 Input control
GSM1089894 Ehf ChIP
BioProject PRJNA191202
SRA SRP019224

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44741_RAW.tar 220.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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