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Series GSE44700 Query DataSets for GSE44700
Status Public on Mar 14, 2013
Title C/EBPα induces highly efficient macrophage transdifferentiation of selected B-lymphoma /leukemia cell lines and impairs their tumorigenicity
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Earlier work demonstrated that the transcription factor C/EBPα can convert immature and mature murine B lineage cells into functional macrophages. Testing >20 human lymphoma and leukemia B-cell lines, we found that most can be transdifferentiated at least partially into macrophage-like cells, provided that C/EBPα is expressed at sufficiently high levels. A tamoxifen-inducible subclone of the Seraphina Burkitt lymphoma line, expressing C/EBPαER, could be efficiently converted into phagocytic and quiescent cells with a transcriptome resembling normal macrophages. The converted cells retained their phenotype even when C/EBPα was inactivated, a hallmark of cell reprogramming. Interestingly, C/EBPα induction also impaired the cells' tumorigenicity. Likewise, C/EBPα efficiently converted a B- lymphoblastic leukemia cell line into macrophage-like cells, again dramatically impairing their tumorigenicity. Our experiments show that human cancer cells can be induced to transdifferentiate by C/EBPα into seemingly normal cells at high frequencies and provide a proof of principle for a potential new therapeutic strategy to treat B-cell malignancies.
 
Overall design Changes in gene expression during transdifferentiation of BLaER1 cells, comparing uninduced (0h) cells with cells treated with E2 for 3h, 6h, 9h, 12h, 18h, 24h, 36h, 48h, 72h, 120h or 168h. Primary human B-cells and macrophages were used as controls. 2 replicates each.
 
Contributor(s) Rapino F, Robles EF, Richter-Larrea JA, Kallin EM, Martinez-Climent JA, Graf T
Citation(s) 23545498
Submission date Feb 26, 2013
Last update date Nov 27, 2018
Contact name Francesca Rapino
Organization name center for genomic regulation
Street address dr.aiguader 88
City Barcelona
ZIP/Postal code 08003
Country Spain
 
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (28)
GSM1089101 BLaER1 0h_1
GSM1089102 BLaER1 0h_2
GSM1089103 BLaER1 3h_1
Relations
BioProject PRJNA192979

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44700_RAW.tar 347.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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