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Series GSE44693 Query DataSets for GSE44693
Status Public on Apr 29, 2014
Title MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation (part 2)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Over-expression of miR-155 induces changes in the pattern of gene expression of hCMEC/D3 cells. hypothesis tested in the present study was that miR-155 constitute an important regulatory control of the brain endothelial response to inflammatory cytokines.
 
Overall design To identify miR-155 target genes in brain endothelim that might be implicated in BBB dysfunction relevant to human disease, we then analysed changes in mRNA expression of hCMEC/D3 cells that overexpress miR-155 and results were contrasted to cells transfected with scrambled miR. To ectopically express miR-155 in hCMEC/D3 cells, 30 nM of pre-miR-155 and the siPORT Amine transfection agent (Applied Biosystems, Warrington, UK) were combined following the manufacturer’s instructions.
 
Contributor(s) Lopez-Ramirez MA, Wu D, Pryce G, Simpson JE, Reijerkerk A, King-Robson J, Kay O, de Vries HE, Hirst MC, Sharrack B, Baker D, Male DK, Michael GJ, Romero IA
Citation(s) 24604078, 27246706
Submission date Feb 26, 2013
Last update date Oct 03, 2019
Contact name Miguel Alejandro Lopez-Ramirez
E-mail(s) malopezramirez@ucsd.edu
Phone 858-822-6487
Organization name University of California, San Diego
Department School of Medicine
Street address 9500 Gilman Drive
City La Jolla
State/province California/San Diego
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (6)
GSM1089046 pre-miR-155 transfected cells Rep1
GSM1089047 pre-miR-155 transfected cells Rep2
GSM1089048 pre-miR-155 transfected cells Rep3
This SubSeries is part of SuperSeries:
GSE44694 MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation
Relations
BioProject PRJNA191031

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44693_RAW.tar 3.9 Mb (http)(custom) TAR
GSE44693_non-normalized.txt.gz 2.1 Mb (ftp)(http) TXT
GSE44693_normalized.txt.gz 983.5 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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