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Series GSE44654 Query DataSets for GSE44654
Status Public on Mar 26, 2013
Title An Ancient Transcription Factor Initiates the Burst of piRNA Production During Early Meiosis in Mouse Testes (RNA-Seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Animal germ cells produce PIWI-interacting RNAs (piRNAs), small silencing RNAs that suppress transposons and enable gamete maturation. Mammalian transposon-silencing piRNAs accumulate early in spermatogenesis, whereas pachytene piRNAs are produced later during post-natal spermatogenesis and account for >95% of all piRNAs in the adult mouse testis. Mutants defective for pachytene piRNA pathway proteins fail to produce mature sperm, but neither the piRNA precursor transcripts nor the trigger for pachytene piRNA production is known. Here, we show that the transcription factor A-MYB initiates pachytene piRNA production. A-MYB drives transcription of both pachytene piRNA precursor RNAs and the mRNAs for core piRNA biogenesis factors, including MIWI, the protein through which pachytene piRNAs function. A-MYB regulation of piRNA pathway proteins and piRNA genes creates a coherent feed-forward loop that ensures the robust accumulation of pachytene piRNAs. This regulatory circuit, which can be detected in rooster testes, likely predates the divergence of birds and mammals.
 
Overall design Transcriptome and ChIP sequencing in mouse and rooster testes
 
Contributor(s) Li XZ, Roy CK, Zamore PD
Citation(s) 23523368
Submission date Feb 25, 2013
Last update date May 15, 2019
Contact name Christian Roy
Organization name Umass Med
Street address 364 Plantation St
City Worcester
State/province Massachusetts
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (14)
GSM1088420 wild.type.42.rnaseq
GSM1088421 wild.type.10.rnaseq
GSM1088422 wild.type.12.rnaseq
This SubSeries is part of SuperSeries:
GSE44690 An Ancient Transcription Factor Initiates the Burst of piRNA Production During Early Meiosis in Mouse Testes
Relations
SRA SRP018855
BioProject PRJNA190912

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44654_101-assembled.transcripts.bed.gz 2.5 Mb (ftp)(http) BED
GSE44654_rnaseq.rpkm.matrix.csv.gz 3.8 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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