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Series GSE44638 Query DataSets for GSE44638
Status Public on Aug 18, 2013
Title Global DNA Methylation Remodeling Accompanies CD8 T Cell Effector Function
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary In response to acute infection CD8 T cells differentiate into effector cells capable of clearing the antigen. While the transcriptional and functional changes have previously been studied little is known of the epigenetic modifications that accompany this differentiation process. To gain insights into CD8 T cell effector differentiation and the role of epigenetics, we mapped DNA methylation by MeDIP-seq in naive CD8 T cells and day 8 effector CD8 T cells that are induced following an acute infection. We identified hundreds of thousands of differentially methylated regions (DMRs). Promoter DNA methylation inversely correlated with gene expression and DMRs were enriched for functional transcription factor binding sites. These data indicated that DNA methylation is dynamic during CD8 T cell differentiation and provide a map of possible regulatory regions important in this process.
Overall design Examination of DNA methylation during CD8 T cell differentiation from naïve to day 8 effectors following acute infection
Contributor(s) Scharer CD, Boss JM
Citation(s) 23956425
Submission date Feb 25, 2013
Last update date May 15, 2019
Contact name Chris Scharer
Organization name Emory University
Department Microbiology and Immunology
Lab Chris Scharer
Street address 1510 Clifton Rd, Suite 3086A
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM1088173 CD8 Naive
GSM1088174 CD8 D8 Effector
GSM1088175 CD8 Input
SRA SRP018844
BioProject PRJNA191093

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44638_CD8.1.5fold.DMRs.bed.gz 5.3 Mb (ftp)(http) BED
GSE44638_RAW.tar 1.6 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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