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Series GSE44595 Query DataSets for GSE44595
Status Public on Feb 23, 2013
Title Role for HA and the viral RNA polymerase complex in the virulence of the 1918 influenza virus
Organism Mus musculus
Experiment type Expression profiling by array
Summary The goal of this experiment was to examine the biological properties of reassortant viruses between the 1918 virus and a contemporary human H1N1 virus to determine the role of hemagglutinin (HA) and the viral RNA polymerase complex of 1918 virus toward pathogenesis and abberant host responses in mice.
Overall design Six- to eight-week-old female BALB/c mice were anesthetized and inoculated with 50 μl of phosphate-buffered saline (PBS; Mock) or with 10^6 pfu of virus in a 50 μl volume. There are a total of six biological conditions: wild-type 1918 virus (A/Brevig Mission/1/1918; 1918), wild-type K173 virus (A/Kawasaki/173/2001; K173), three reassortant viruses between 1918 and K173 viruses [1918HA/K173, 1918PB1/K173, and 1918(3P+NP)/K173], and mock-infected control animals. Nine animals per virus group were inoculated, and whole lungs were collected at 1, 3 and 5 days post-inoculation from three animals per virus group per time point. Two of three mice inoculated with 1918 virus and 1918HA/K173 virus died by day 5 post-inoculation. Six animals were inoculated with PBS. Whole lungs from two mock-infected animals per time point were collected and served as the uninfected reference. All samples were processed for lung gene expression analysis using oligonucleotide microarrays. Technical replicates were performed for 9 samples.
Contributor(s) Watanabe T, Go JT, Watanabe S, Shinya K, Tchitchek N, Benecke A, Katze MG, Kawaoka Y
Citation(s) 23449804
Submission date Feb 22, 2013
Last update date Jan 12, 2017
Contact name Michael Katze
Organization name University of Washington
Department Microbiology
Lab Michael G. Katze, Ph.D
Street address Rosen Building 960 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109-4325
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (56)
GSM1087542 1918(3P+NP)/K173_D1_A1
GSM1087543 1918(3P+NP)/K173_D1_A2
GSM1087544 1918(3P+NP)/K173_D1_A3
BioProject PRJNA190605

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Supplementary file Size Download File type/resource
GSE44595_RAW.tar 504.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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