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Series GSE44549 Query DataSets for GSE44549
Status Public on Feb 21, 2013
Title Genomic changes in low vs high metastatic A549 NSCLC cell line
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
SNP genotyping by SNP array
Summary Genomic changes in low and highly metastatic A549 cells were analyzed by 500K SNP arrays. A large number of genomic alterations were present in A549 cells but no significant differences were observed between the low or highly metastatic A549 cell lines.
Overall design We generated a NSCLC line with highly increased propensity to form tumor nodules in murine lungs after intravenous injections. Extravasation and growth at a distant site are important parts of the metastatic process and we regarded these as a surrogate marker for in vivo aggressiveness and potential metastatic capability. A549 lung asdenocarcimona cell line with initially low metastatic potential was used for this purpose; these cells formed multiple small nodules in NOD/SCID mice after first i.v.-injection, round 1 (R1). Removal of tumor nodules from the lungs and subsequent re-injection led to a rapid increase in metastatic capacity. A highly aggressive phenotype which was stable over time was evident after three rounds (R3) of in vivo selection for the A549 cell line.
Contributor(s) Thoennissen NH
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Submission date Feb 21, 2013
Last update date Nov 27, 2018
Contact name Nils H Thoennissen
Organization name University Hospital of Münster
Department Hematology Oncology
Street address Albert-Schweitzer-Str. 33
City Münster
ZIP/Postal code 48149
Country Germany
Platforms (1)
GPL6801 [GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array
Samples (2)
GSM1086289 A549 cell line, round 0, before in vivo injection
GSM1086290 A549 cell line, round 3, after 3 times in vivo injection
BioProject PRJNA190053

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44549_RAW.tar 104.6 Mb (http)(custom) TAR (of CEL, CFA, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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