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Series GSE44412 Query DataSets for GSE44412
Status Public on Apr 08, 2014
Title Holocentromeres are dispersed point centromeres localized at transcription factor hotspots
Organism Caenorhabditis elegans
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The centromere is a defining feature of eukaryotic chromosomes and is essential for the segregation of chromosomes during cell division. Centromeres are universally marked by the histone variant cenH3 and are restricted to specialized chromatin that most commonly localized to a single position along the chromosome. However, the DNA on which centromeric nucleosomes assemble is not conserved and varies greatly in size and composition. It ranges from genetically defined point centromeres that assemble a single cenH3-containing nucleosome to epigenetically defined regional centromeres embedded in megabases of tandemly repeated DNA to holocentromeres that extend along the length of the entire chromosomes. The organization of regional and holocentric centromeres has so far been elusive, as the precise locations of cenH3-containing sequences could not be determined. Our results show that the point centromere is the basic unit of holocentromeres and provide a basis for understanding how centromeric chromatin is maintained.
Overall design We use high-resolution mapping of cenH3-associated DNA to show that Caenorhabditids elegans holocentromeres are organized as dispersed but discretely localized point centromeres.
Contributor(s) Steiner FA, Henikoff S
Citation(s) 24714495
Submission date Feb 19, 2013
Last update date May 15, 2019
Contact name Jorja Henikoff
Phone 206-667-4850
Organization name Fred Hutchinson Cancer Research Center
Department Basic Sciences
Lab Henikoff
Street address 1100 Fairview AV N, A1-162
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
Platforms (2)
GPL13657 Illumina HiSeq 2000 (Caenorhabditis elegans)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
Samples (19)
GSM1089264 WT_CenH3_10min_(20110706_8)
GSM1089265 WT_CenH3_1min_(20110706_5)
GSM1089266 WT_CenH3_2min_rep1_(20110706_6)
BioProject PRJNA191143
SRA SRP018884

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44412_MNase_ChIP_Seq_protocol_v2.pdf.gz 80.2 Kb (ftp)(http) PDF
GSE44412_RAW.tar 58.4 Gb (http)(custom) TAR (of BAM, BED, BEDGRAPH, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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