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Series GSE44292 Query DataSets for GSE44292
Status Public on Mar 24, 2014
Title Gene Expression data from mouse bone marrow derived macrophages treated with different inflammatory stimuli
Organism Mus musculus
Experiment type Expression profiling by array
Summary The activation profiles of macrophages under different immune and inflammatory conditions have generated great interest. LPS, in particular, is a commonly used in vitro model of infection and inflammation studies in macrophages. We have used gene expression microarrays to define the effects of each of three variables; LPS dose, LPS vs. interferons beta and gamma, and genetic background on the transcriptional response of mouse bone marrow-derived macrophages
Macrophages derived from the C57BL/6 strain of mouse were challenged with increasing doses of LPS over a 24 hour time-course (0.5ng/ml, 5ng/ml, or 50ng/ml LPS). BALB/c derived macrophages were treated with any of 5ng/ml LPS, 10U/ml of recombinant mouse interferon-beta, or 10U/ml interferon-gamma, over a 24 hour timecourse. Sampling times were 0 hours (pre-treatment) and 1, 2, 4, 8, and 24 hours post-treatment in each case.
Overall design 64 samples in total were analysed. These incorporated 6 different timecourse studies. A combination of statistical filtering using the Empirical Bayes function in Bioconductor package (R statistical software), and co-expression analysis using the network analysis tool BioLayout Express 3D, was used to compare the timecourse studies.
Web link
Contributor(s) Raza S, Freeman TC, Hume DA
Citation(s) 24721704, 24962162
Submission date Feb 13, 2013
Last update date Jan 08, 2020
Contact name Sobia Raza
Organization name The Roslin Institute
Department The University of Edinburgh
Street address Easter Bush Campus
City Midlothian
ZIP/Postal code EH25 9RG
Country United Kingdom
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (64)
GSM1082462 0h_Con-1_BALBc
GSM1082463 0h_Con-2_BALBc
GSM1082464 1h_Ifnb-1_BALBc
BioProject PRJNA189329

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44292_RAW.tar 282.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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