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Series GSE44133 Query DataSets for GSE44133
Status Public on Jan 01, 2015
Title Tobacco-induced mRNAs profile alterations in human spermatozoa: a preliminary study for further knowledge of toxical spermatogenesis impairment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Tobacco smoking generates deleterious effects on human semen quality but mechanisms by which cigarettes smoking can impact spermatogenesis are poorly understood. Recent works have shown that spermatozoa RNAs can be used to understand mechanisms involved in tobacco induced spermatogenesis impairment. We performed a prospective study of 8 smoker and 8 non-smoker patients in an university hospital. All patients were selected according to an occupational exposure standardized questionnaire and the sperm parameters. We performed gene expression and miRNA microarrays using RNA extracts from spermatozoa of 8 smokers and 8 non-smokers. Quantification of selected miRNA was performed using quantitative RT-PCR.
We show that 16 genes were differentially expressed between smokers and non-smokers, of which 5 were upregulated and 11 were down regulated in smokers. 23 microRNAs were differentially expressed, of which 16 were upregulated and 7 were down regulated in smokers. Quantitative RT-PCR confirmed the down regulation in smokers for 3 microRNAs. Moreover in smokers, one of the upregulated genes is a putative target for one down regulated microRNA.

This is a preliminary and innovating study on spermatozoa RNA extracts in the field of infertility. This preliminary study shows that large scale approaches are non invasive diagnostic tools that may help elucidate the mechanisms that mediate the response to tobacco smoke exposure on human spermatogenesis. This could be used to design biomarkers of human spermatogenetic damage, and help elucidate the fine regulatory mechanisms that mediate responses to environmental agent exposure during human spermatogenesis.
 
Overall design Total RNAs were extracted from spermatozoa of 11 patients including: 5 non-smokers and 6 smokers
 
Contributor(s) Metzler-Guillemain C, Lepoivre C, Victorero G, Bergon A, Perrin J, Sari-Minodier I, Boulanger N, Rihet P, Nguyen C
Citation(s) 25821920
Submission date Feb 06, 2013
Last update date Feb 22, 2018
Contact name Aurelie Bergon
E-mail(s) aurelie.bergon@inserm.fr
Phone +33(0) 491 828 724
Organization name TAGC INSERM U1090
Street address parc scientifique de Luminy, case 928
City MARSEILLE CEDEX 09
ZIP/Postal code 13 288
Country France
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (11)
GSM1079529 Spermatozoa_S1_rep1
GSM1079530 Spermatozoa_NS1_rep1
GSM1079531 Spermatozoa_S2_rep1
This SubSeries is part of SuperSeries:
GSE44135 Tobacco-induced microRNAs and mRNAs profile alterations in human spermatozoa: a preliminary study for further knowledge of toxical spermatogenesis impairment
Relations
BioProject PRJNA188743

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44133_RAW.tar 105.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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