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Series GSE44119 Query DataSets for GSE44119
Status Public on Apr 01, 2013
Title The histone chaperone Spt6 coordinates histone H3K27 demethylation to regulate gene expression and myogenesis
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Histone chaperones affect chromatin structure and gene expression through interaction with histones and RNA polymerase II (PolII). Here, we report that the histone chaperone Spt6 counteracts H3K27me3, an epigenetic mark deposited by the Polycomb Repressive Complex 2 (PRC2) and associated with transcriptional repression. We found that Spt6 is required for proper engagement and function of the H3K27 demethylase KDM6A (UTX) on muscle genes and regulates muscle gene expression and cell differentiation. ChIP-Seq experiments revealed an extensive genome-wide overlap of Spt6, PolII and KDM6A at transcribed regions that are devoid of H3K27me3. Mammalian cells and zebrafish embryos with reduced Spt6 display increased H3K27me3 and diminished expression of the master regulator MyoD, resulting in myogenic differentiation defects. As a confirmation for an antagonistic relationship between Spt6 and H3K27me3, inhibition of PRC2 permits MyoD re-expression in myogenic cells with reduced Spt6. Our data indicate that, through cooperation with PolII and KDM6A, Spt6 orchestrates removal of H3K27me3, thus effectively controlling developmental gene expression and cell differentiation.
Overall design Examination of Spt6 and KDM6A levels in a skeletal muscle cells at various developmental stages
Contributor(s) Wang AH, Zare H, Mousavi K, Sartorelli V
Citation(s) 23503590
Submission date Feb 06, 2013
Last update date May 15, 2019
Contact name Hossein Zare
Organization name NIH
Street address 5o South Dr #1347
City Bethesda
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (5)
GSM1079204 MB_Spt6_ChIPSeq
GSM1079205 MT_Spt6_ChIPSeq
GSM1079206 MB_KDM6A_ChIPSeq
BioProject PRJNA188719
SRA SRP018432

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Supplementary file Size Download File type/resource
GSE44119_RAW.tar 1.0 Gb (http)(custom) TAR (of BED, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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