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Series GSE44060 Query DataSets for GSE44060
Status Public on Oct 23, 2013
Title Expression profiling of Troy positive gastric cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Proliferation of the self-renewing epithelium of the gastric corpus occurs almost exclusively in the isthmus of the glands, from where cells migrate bi-directionally towards pit and base. The isthmus is therefore generally viewed as the stem cell zone. We find that the stem cell marker Troy is expressed at the gland base by a small subpopulation of chief cells. By lineage tracing using a Troy-eGFP-ires-CreERT2 allele, single marked cells are shown to generate entirely labeled gastric units over periods of months. This phenomenon accelerates upon tissue damage. Troy+ chief cells can be cultured to generate long-lived gastric organoids. Troy marks a specific, 'plastic' subset of differentiated chief cells capable of replenishing entire gastric units, essentially serving as a quiescent ‘reserve’ stem cell.
Overall design An EGFP-ires-CreERT2 cassette was introduced into the transcriptional start side of Tnfrsf19 (Troy), resulting in the expression of EGFP and CreERT2 under the control of endogenous Troy-regulatory sequences. Troy is expressed in basally located chief and parietal cells. Using FACS, Troy positive chief and parietal cells can be isolated separately. Expression profiling was performed on these two cell types: three arrays of sorted chief cells, two arrays of sorted parietal cells. As a reference, two arrays of separately isolated whole gastric corpus glands were added.
In addition, single sorted Troy positive chief cells can be placed in culture, forming 3D cultures called organoids. Three arrays were generated from different organoid cultures, one time in normal medium containing the factors EGF, Gastrin, FGF10, Noggin, Wnt3a and Rspondin (EGFNWR medium) and one time in medium containing only EGF and Rspondin (ER medium).
Contributor(s) Stange DE, Clevers H
Citation(s) 24120136
Submission date Feb 05, 2013
Last update date Apr 18, 2017
Contact name Daniel E. Stange
Organization name University Clinic Dresden
Street address Fetscherstr. 74
City Dresden
ZIP/Postal code 01307
Country Germany
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (13)
GSM1077412 Troy_corpus_chief_a
GSM1077413 Troy_corpus_chief_b
GSM1077414 Troy_corpus_chief_c
BioProject PRJNA188502

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44060_RAW.tar 58.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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