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Series GSE43932 Query DataSets for GSE43932
Status Public on Feb 01, 2014
Title Commensal microbiota contributes to chronic endocarditis and arrhythmia in TAX1BP1 deficient mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Tax1-binding protein 1 (Tax1bp1) negatively regulates NF-κB by editing the ubiquitylation of target molecules with its catalytic partner A20. Genetically engineered TAX1BP1-deficient (KO) mouse develops age-dependent inflammatory constitution in multiple organs including heart, liver, skin and succumb to premature heart failure. Laser capture dissection and gene expression microarray analysis on the mitral valves of TAX1BP1-KO mice (8 and 16 week old) revealed that the 588 transcription alterations. SAA3 (serum amyloid A3), at 1,180-fold induction (FI), CHI3L1(361-FI), HP(187-FI), IL1B(122-FI) and SPP1/OPN(101-FI) and WIF1 (Wnt inhibitory factor 1) at 11-fold decrease implied extensive inflammation and tissue degeneration at this microenvironment. Intense Saa3 staining and significant reduction of I-κBα at the same area, massive infiltration of inflammatory lymphocytes and edema formation at the peripheral areas of sinoatrial and atrioventricular node were also observed and electrocardiogram indicated atrioventricular conduction defect (elongated PQ-interval) in TAX1BP1-KO mice. Since antibiotics-induced ‘germ free’ status for three months significantly ameliorated these chronic autoimmune and altered cardiac excitation properties, we conclude that these chronic pathological conditions, as we named ‘pseudo-infective endocarditis’ were boosted by the commensal microbiota those who are usually harmless by their nature. This experimental outcome raises a novel mechanistic linkage between endothelial inflammation and cardiac dysfunction.
 
Overall design We employed laser capture microdissection (LCM) and gene expression microarray technique to obtain the specific gene expression information of pathologic organ. Total RNAs of mitral valves from three independent tissues of 8- or 16-week age (-wk) male mice of either WT or TAX1BP1-KO mice were prepared by LCM followed by total RNA extraction kit. Then the global mRNA expression profiles were analyzed by Agilent gene expression microarray.
 
Contributor(s) Nakano S, Ikebe E, Tsukamoto Y, Wang Y, Matsumoto T, Mitsui T, Yahiro T, Inoue K, Kawazato H, Yasuda A, Ito K, Yokoyama S, Tanaka Y, Hasegawa H, Jeang K, Hori M, Shimada T, Moriyama M, Ono K, Kubota T, Fujibuchi W, Iha H, Nishizono A
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Submission date Jan 30, 2013
Last update date Jan 12, 2017
Contact name Satoko Nakano
E-mail(s) sanakano@oita-u.ac.jp
Organization name Oita University
Street address 1-1 Idaigaoka Yufu
City Oita
ZIP/Postal code 879-5593
Country Japan
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM1074546 TAX1BP1_8w_KO_mitral valves_1
GSM1074547 TAX1BP1_8w_KO_mitral valves_2
GSM1074548 TAX1BP1_8w_KO_mitral valves_3
Relations
BioProject PRJNA188554

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43932_RAW.tar 22.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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