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Series GSE43879 Query DataSets for GSE43879
Status Public on Feb 11, 2013
Title An intricate interplay between astrocytes and motor neurons in ALS
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Amyotrophic Lateral Sclerosis (ALS) results from the selective and progressive degeneration of motor neurons. Although the underlying disease mechanisms remain unknown, glial cells have been implicated in ALS disease progression. Here we examine the effects of glial cell/motor neuron interactions on gene expression, using the hSOD1G93A mouse model of ALS. We detect striking cell autonomous and non-autonomous changes in gene expression in co-cultured motor neurons and glia, revealing that the two cell types profoundly affect each other. In addition, we found a remarkable concordance between the cell culture data, expression profiles of whole spinal cords, and of acutely isolated spinal cord cells, during disease progression in the G93A mouse model, providing validation of the cell culture approach. Bioinformatics analyses identified changes in the expression of specific genes and signaling pathways that may contribute to motor neuron degeneration in ALS, among which are TGF-b signaling pathways.
Overall design RNA-seq profiles of: 1) 43 Sandwich culture samples at 3 different time points (3, 7 and 14 days), in duplicate, in different combinations of genetic background WT/SOD1_G93A mutant glia and WT/SOD1_G93A mutant neurons; 2) 16 spinal cord samples at 4 different time points, WT and SOD1_G93A mutant.
Contributor(s) Phatnani HP, Maniatis T
Citation(s) 23388633
Submission date Jan 29, 2013
Last update date May 15, 2019
Contact name Sean O'Keeffe
Organization name Columbia University
Street address 701 West 168th Street
City New York
ZIP/Postal code 10032
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (59)
GSM1072754 12wk_MT_F [spinal cord]
GSM1072755 12wk_MT_M [spinal cord]
GSM1072756 12wk_WT_F [spinal cord]
BioProject PRJNA187985
SRA SRP018287

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Supplementary file Size Download File type/resource
GSE43879_sandwich.txt.gz 4.8 Mb (ftp)(http) TXT
GSE43879_spinalCord.txt.gz 3.7 Mb (ftp)(http) TXT
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