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| Status |
Public on Mar 05, 2014 |
| Title |
Long non-coding RNA HOTAIR is expressed in small cell lung cancer and is relevant to cellular proliferation, invasiveness and clinical relapse: tissue and cell-line analyses |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
LincRNA HOTAIR was expressed in pure SCLC and higher expression was significantly related to lymphatic invasion and relapse. Multivariate analyses demonstrated that HOTAIR expression correlated with RFS. In vitro experiments demonstrated that half of SCLC cell lines expressed HOTAIR at higher levels than normal cells and that, using cells of SBC-3, knockdown of HOTAIR decreased proliferative activity and cellular invasiveness with altered expression of cell adhesion-related genes.
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| Overall design |
Total RNAs from one siHOTAIR-transfected SBC-3 cells and control cells (siGFP-transfected cells), were extracted using RNeasy mini kit Plus (Qiagen) and hybridized to the microarrays, Sure Print G3 Human GE 8x60K microarrays (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions. Subsequently, data analysis was carried out using the GeneSpring GX 12 software (Agilent Technologies), with a stringency of P<0.1 and a 2-fold or more change using gene ontology analysis.
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| Contributor(s) |
Ono H, Ishukawa Y |
| Citation(s) |
24591352 |
| Submission date |
Jan 29, 2013 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Hiroshi Ono |
| E-mail(s) |
hono.pulm@tmd.ac.jp
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| Organization name |
The Cancer Institute of JFCR
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| Department |
Pathology
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| Street address |
3-8-31 Ariake, Koto-ku
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| City |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA187984 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43877_RAW.tar |
11.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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