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Series GSE43877 Query DataSets for GSE43877
Status Public on Mar 05, 2014
Title Long non-coding RNA HOTAIR is expressed in small cell lung cancer and is relevant to cellular proliferation, invasiveness and clinical relapse: tissue and cell-line analyses
Organism Homo sapiens
Experiment type Expression profiling by array
Summary LincRNA HOTAIR was expressed in pure SCLC and higher expression was significantly related to lymphatic invasion and relapse. Multivariate analyses demonstrated that HOTAIR expression correlated with RFS. In vitro experiments demonstrated that half of SCLC cell lines expressed HOTAIR at higher levels than normal cells and that, using cells of SBC-3, knockdown of HOTAIR decreased proliferative activity and cellular invasiveness with altered expression of cell adhesion-related genes.
Overall design Total RNAs from one siHOTAIR-transfected SBC-3 cells and control cells (siGFP-transfected cells), were extracted using RNeasy mini kit Plus (Qiagen) and hybridized to the microarrays, Sure Print G3 Human GE 8x60K microarrays (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions. Subsequently, data analysis was carried out using the GeneSpring GX 12 software (Agilent Technologies), with a stringency of P<0.1 and a 2-fold or more change using gene ontology analysis.
Contributor(s) Ono H, Ishukawa Y
Citation(s) 24591352
Submission date Jan 29, 2013
Last update date Nov 27, 2018
Contact name Hiroshi Ono
Organization name The Cancer Institute of JFCR
Department Pathology
Street address 3-8-31 Ariake, Koto-ku
City Tokyo
ZIP/Postal code 135-8550
Country Japan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (2)
GSM1072712 #1siHOTAIR transfected SBC3
GSM1072713 siGFP transfected SBC3
BioProject PRJNA187984

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43877_RAW.tar 11.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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