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Series GSE43810 Query DataSets for GSE43810
Status Public on Dec 31, 2014
Title Phenotypic and genotypic stability in a preclinical model of renal cell carcinoma
Organism Homo sapiens
Experiment type Genome variation profiling by genome tiling array
Summary Renal cell carcinomas are often resistant to conventional cytotoxic agents. Xenograft models are used for in vivo preclinical studies and drug development. The validity of these studies is highly dependent on the phenotypic and genotypic stability of the models. We evaluated the stability of six kidney tumors xenografted in nude/NMRI mice. We compared the initial sample (P0), 1st (P1) and 5th (P5) passages for the following criteria: histopathological analysis, immunohistochemistry for CK7, CD10, vimentin and p53, and DNA analysis with 10 microsatellites. We also performed CGH-array and we compared the sensitivity to sunitinib with tumor response in patients. The six xenografted tumors showed no difference between the morphology of the primary tumor and that of the xenografted tumor at P1 and P5 but we observed differences for CK7 expression in one case and for p53 expression in another. Out of 44 P0-P1-P5 groups with fully available microsatellite data, 29 (66%) showed no significant difference from P0 to P5 while 15 (34%) showed new or lost alleles. In CGH-array, genomic alterations at P5 were close to those already present in the initial tumor. We also observed similarity between the models and the patients for response to antiangiogenic treatment. We showed that the six xenograft models were phenotypically and genotypically stable across passages and showed similarity with response to treatment in patients.
 
Overall design CGH-arrays are realized on samples at P0 (samples K8-614_P0 and K9-162_P0) and their corresponding xenografted tumors at P5 (samples K8-614_P5 and K9-162_P5)
 
Contributor(s) Varna M, Bousquet G, Ferreira I, Goulard M, El-Bouchtaoui M, Artus PM, Verine J, de Kerviler E, Hernandez L, Leboeuf C, Escudier B, Legrès L, Setterblad N, Soliman H, Feugeas J, Janin A, Bertheau P
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Submission date Jan 28, 2013
Last update date Dec 31, 2014
Contact name Anne Janin
E-mail(s) jean-paul.feugeas@sls.aphp.fr
Phone +33142494012
Organization name University Paris 7, INSERM, APHP
Street address 1 Av C Vellefaux
City Paris
ZIP/Postal code 75010
Country France
 
Platforms (1)
GPL10123 Agilent-022060 SurePrint G3 Human CGH Microarray 4x180K (Feature Number version)
Samples (4)
GSM1071622 K8-614 P0
GSM1071623 K8-614 P5
GSM1071624 K9-162 P0
Relations
BioProject PRJNA187489

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43810_RAW.tar 72.7 Mb (http)(custom) TAR (of TXT)
GSE43810_normalized.txt.gz 3.9 Mb (ftp)(http) TXT
Processed data are available on Series record

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