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| Status |
Public on Apr 28, 2013 |
| Title |
The interactome of POU5F1 upstream enhancer in mouse embryonic stem cells revealed by 4C-Seq |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
We report the application of 4C-Seq technique for exploring POU5F1 enhancer interactome in mouse embryonic stem cells. A statistical model was built to identify enriched interacting regions from raw 4C data. The biological replicate data were compared to identify reproducible interacting regions. The interacting sites in the reproducible regions are enriched with active histone marks as well as transcription factors Oct4, Klf4, Esrrb, Tcfcp2i1 and Zfx that are critical for reprogramming and pluripotency.
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| |
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| Overall design |
Generation of Illumina HiSeq2000 sequencing data using 4C-Seq protocol
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| |
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| Contributor(s) |
Gao F, Wei Z, Wang K, Lu W |
| Citation(s) |
23705979 |
| Submission date |
Jan 25, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Fan Gao |
| E-mail(s) |
gaofan83@hotmail.com
|
| Organization name |
USC
|
| Department |
ZNI
|
| Street address |
1501 San Pablo St. ZNI 245
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA187390 |
| SRA |
SRP018230 |
