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Series GSE43674 Query DataSets for GSE43674
Status Public on Dec 02, 2013
Title U87 cells implanted on a chick CAM (Chorioallantoic Membrane)
Organisms Gallus gallus; Homo sapiens
Experiment type Expression profiling by array
Summary Glioblastoma multiforme (GBM) brain tumours have one of the shortest mean survival times (<1 year). In vivo models of GBM in mice and rats have been developed to study aspects of glioma that cannot be observed in cell culture such as angiogenesis, invasion and metastasis. Gliomas can be induced by implantation of rodent glioma cell lines into the brain or flank of nude mice. The disadvantages of rodent models however include variable growth rate and poor penetrance, which leads to difficulties in collecting clearly graded samples (pre-vascular/vascular). Bikfalvi et al have previously established a human GBM model that addresses these issues based on the chicken egg chorio-allantoic membrane (CAM), a highly vascularised extra-embryonic tissue. We have used DNA microarrays and a CAM model of GBM to study gene expression during the recruitment and development of the tumour vasculature. Over a 5 day period samples were taken every 12 hours from the tumour implantation site consisting of tumour cells and stroma cells, and also from a site distant from the implantation site consisting of just CAM cells. This study will shed light on the dynamic transcriptional signature of pathological angiogenesis.
Overall design On day 10 of embryonic development 3-5 million U87 cells were deposited onto the surface of the CAM after gentle laceration. The cells were contained within a plastic ring and each tumour was size matched based on its volume. Tumour/stroma and distant CAM samples were cut out at 12 hour intervals post implantation for 5 days, equalling 10 time points. Each time point consisted of three replicates. U87 cells in culture (pre-implantation) were also included in the study in triplicate.
Contributor(s) Clarke K
Citation(s) 26132659
Submission date Jan 23, 2013
Last update date Aug 09, 2019
Contact name Nil Turan
Organization name University of Birmingham
Street address University of Birmingham, Edgbaston
City Birmingham
ZIP/Postal code B15 2TT
Country United Kingdom
Platforms (2)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
GPL16532 Agilent-023616 Custom Gallus gallus Array
Samples (97)
GSM1067933 TUM D11 MORN 1
GSM1067934 TUM D11 MORN 2
GSM1067935 TUM D11 MORN 3
BioProject PRJNA187039

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43674_RAW.tar 213.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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