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| Status |
Public on Nov 23, 2015 |
| Title |
Dynamics of TRIM33 binding in macrophages and effects of TRIM33 deletion on chromatin state during activation of primary macrophages. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To get insight into TRIM33 functions, TRIM33 ChIP-seq was carried out in murine macrophage cell line (RAW) and in bone marrow-derived macrophages (BMDM). The results showed that, in addition to its role in hematopoietic differentiation, TRIM33 may modulate PU.1 transcriptional activity during macrophage development and/or activation.To characterize the role of TRIM33 in macrophages, we bred TRIM33fl/fl mice with Lyz-Cre mice where the Cre recombinase gene is under the regulatory sequences of the Lyz gene that is expressed only in mature myeloid cells. Bone marrow cells from LyzCre/Trim33+/+ mice and LyzCre/Trim33flox/flox mice were differentiated in macrophages and treated during 0h, 4h, 12h and 24h with LPS. Using ChIP-seq, we provide a link between TRIM33 binding and H3K4me3 spreading on inflammatory genes in macrophages.
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| Overall design |
Chromatin immunoprecipitations of TRIM33 and H3K4Me3 followed by multiparallel sequencing performed in murine bone marrow-derived macrophages (BMDM).
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| Contributor(s) |
Ferri F, Romeo PH |
| Citation(s) |
26592194 |
| Submission date |
Jan 22, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
federica ferri |
| E-mail(s) |
federica.ferri@cea.fr
|
| Organization name |
cea
|
| Street address |
18 route du panorama
|
| City |
Fontenay aux roses |
| ZIP/Postal code |
92265 |
| Country |
France |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA186942 |
| SRA |
SRP018092 |
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