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Series GSE43654 Query DataSets for GSE43654
Status Public on Nov 23, 2015
Title Dynamics of TRIM33 binding in macrophages and effects of TRIM33 deletion on chromatin state during activation of primary macrophages.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To get insight into TRIM33 functions, TRIM33 ChIP-seq was carried out in murine macrophage cell line (RAW) and in bone marrow-derived macrophages (BMDM). The results showed that, in addition to its role in hematopoietic differentiation, TRIM33 may modulate PU.1 transcriptional activity during macrophage development and/or activation.To characterize the role of TRIM33 in macrophages, we bred TRIM33fl/fl mice with Lyz-Cre mice where the Cre recombinase gene is under the regulatory sequences of the Lyz gene that is expressed only in mature myeloid cells. Bone marrow cells from LyzCre/Trim33+/+ mice and LyzCre/Trim33flox/flox mice were differentiated in macrophages and treated during 0h, 4h, 12h and 24h with LPS. Using ChIP-seq, we provide a link between TRIM33 binding and H3K4me3 spreading on inflammatory genes in macrophages.
Overall design Chromatin immunoprecipitations of TRIM33 and H3K4Me3 followed by multiparallel sequencing performed in murine bone marrow-derived macrophages (BMDM).
Contributor(s) Ferri F, Romeo PH
Citation(s) 26592194
Submission date Jan 22, 2013
Last update date May 15, 2019
Contact name federica ferri
Organization name cea
Street address 18 route du panorama
City Fontenay aux roses
ZIP/Postal code 92265
Country France
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (10)
GSM1067639 TRIM33_RAW
GSM1067640 H3K4me3_WT_0h
GSM1067641 H3K4me3_WT_4h
BioProject PRJNA186942
SRA SRP018092

Download family Format
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Supplementary file Size Download File type/resource
GSE43654_RAW.tar 1.1 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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