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Series GSE43650 Query DataSets for GSE43650
Status Public on May 01, 2014
Title Cell cycle dependence of neuroprogenitor fate determination regulated by Trrap-mediated histone acetylation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Epigenetic control of neural stem/progenitor cell fate is fundamental to achieve a fully brain architecture. Two intrinsic programs regulate neurogenesis, one by epigenetic-mediated gene transcription and another by cell cycle control. Whether and how these two are coordinated to determine temporally and spatially neural development remains unknown. Here we show that deletion of Trrap (Transcription translation associated protein), an essential cofactor for HAT (histone acetyltransferase), leads to severe brain atrophy due to a combination of cell death and a blockade of neuron production. Specifically, Trrap deletion forces differentiation of apical progenitor (AP) fate into basal progenitors (BP) and neurons thereby limiting the total neurogenic production. Despite Trrap’s general role in transcriptional regulation, a genome-wide transcriptome analysis of neuroprogenitors identified the cell cycle regulators that are specifically affected by Trrap deletion. Furthermore, E2F-dependent recruitment of HAT and transcription factors to the promoter of cell cycle regulators is impaired in Trrap-deleted neuroprogenitors. Consistent with these molecular changes, Trrap deletion lengthens particularly G1 and S phases in APs in vivo. Therefore, our study reveals an essential and a distinct function of Trrap-HAT in regulation of cell cycle progression that is required for proper determination of neuroprogenitor fate.
Overall design Determine gene transcriptions by comparing Trrap-deleted and wild type samples
Contributor(s) Wang Z
Citation(s) 24792116
Submission date Jan 22, 2013
Last update date May 15, 2019
Contact name Zechen Chong
Organization name Beijing Institute of Genomics
Street address NO. 7 Beitucheng West Road
City Beijing
ZIP/Postal code 100029
Country China
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1067618 wild
GSM1067619 Trrap knockout
BioProject PRJNA186929
SRA SRP018090

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Supplementary file Size Download File type/resource
GSE43650_gene_exp_diff_sig.txt.gz 114.5 Kb (ftp)(http) TXT
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