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Series GSE43543 Query DataSets for GSE43543
Status Public on Aug 31, 2013
Title Mediator directs co-transcriptional heterochromatin assembly by RNAi-dependent and -independent pathways.
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by array
Summary Background: Heterochromatin at the pericentromeric repeats in fission yeast is assembled and spread by an RNAi-dependent mechanism, which is coupled to the transcription of non-coding RNA from the repeats by RNA polymerase II. In addition, Rrp6, a component of the nuclear exosome, also contributes to heterochromatin assembly and is coupled to non-coding RNA transcription. The multi-subunit complex Mediator, which directs initiation of RNA polymerase II-dependent transcription, has recently been suggested to function after initiation in processes such as elongation of transcription and splicing. However, the role of Mediator in the regulation of chromatin structure is not well understood.

Results: We investigated the role of Mediator in pericentromeric heterochromatin formation and found that deletion of specific subunits of the head domain of Mediator compromised heterochromatin structure. The Mediator head domain was required for Rrp6-dependent heterochromatin nucleation at the pericentromere and for RNAi-dependent spreading of heterochromatin into the neighboring region. In the latter process, Mediator appeared to contribute to efficient processing of siRNA from transcribed non-coding RNA, which was required for efficient spreading of heterochromatin. Furthermore, the head domain directed efficient transcription in heterochromatin.

Conclusions: These results reveal a pivotal role for Mediator in multiple steps of transcription-coupled formation of pericentromeric heterochromatin. This observation further extends the role of Mediator to co-transcriptional chromatin regulation.
 
Overall design Gene expression profile at exponentially-growing phase.in the fission yeast deletion mutants of pmc6, med20 and dcr1.
 
Contributor(s) Oya E, Kato H, Chikashige Y, Tsutumi C, Hiraoka Y, Murakami Y
Citation(s) 23966866
Submission date Jan 16, 2013
Last update date Jan 23, 2023
Contact name Atsushi Matsuda
Organization name National Institute of Information and Communications Technology
Street address 588-2, Iwaoka, Iwaoka-cho, Nishi-ku
City Kobe
ZIP/Postal code 651-2492
Country Japan
 
Platforms (1)
GPL15360 Agilent-028936 KARC-EX-2010-31 026058 [ProbeName version]
Samples (14)
GSM1065023 S.pombe-wildtype-for-dcr1D-rep1
GSM1065024 S.pombe-wildtype-for-dcr1D-rep2
GSM1065025 S.pombe-dcr1D-rep1
Relations
BioProject PRJNA186678

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43543_RAW.tar 36.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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