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Series GSE43540 Query DataSets for GSE43540
Status Public on Mar 02, 2013
Title H3K4me3 Interactions with TAF3 Regulate Preinitiation Complex Assembly and Selective Gene Activation [HIT-Seq]
Organism Mus musculus
Experiment type Other
Summary Histone modifications regulate chromatin-dependent processes, yet the mechanisms by which they contribute to specific outcomes remain unclear. H3K4me3 is a prominent histone mark that is associated with active genes and promotes transcription through interactions with effector proteins that include initiation factor TFIID. We demonstrate that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that (i) H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex (PIC) formation; (ii) H3K4me3, through TAF3 interactions, can act either independently or cooperatively with the TATA box to direct PIC formation and transcription; and (iii) H3K4me3-TAF3/TFIID interactions regulate gene-selective functions of p53 in response to genotoxic stress. Our findings indicate a mechanism by which H3K4me3 directs PIC assembly for the rapid induction of specific p53 target genes
Overall design Examination of genome wide binding sites of TAF3 full length protein vs TAF3 PHD domain alone (Full vs PHD), with or without M880A mutation (WT vs mut) in mouse MEF cells using HITseq method (PNAS 2010, 107:3135-3140, PMID: 20133638)
Contributor(s) Lauberth SM, Nakayama T, Wu X, Ferris A, Hughes SH, Roeder RG
Citation(s) 23452851
Submission date Jan 16, 2013
Last update date May 15, 2019
Contact name Xiaolin Wu
Phone 301-846-7677
Organization name Frederick National Laboratory for Cancer Research/SAIC-Frederick Inc
Department Cancer Research Technology Program
Lab Genomics Laboratory
Street address 8560 Progress Drive, C3001
City Frederick
State/province MD
ZIP/Postal code 21701
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (4)
GSM1065011 TAF3_Full_WT
GSM1065012 TAF3_Full_Mut
GSM1065013 TAF3_PHD_WT
This SubSeries is part of SuperSeries:
GSE43542 H3K4me3 Interactions with TAF3 Regulate Preinitiation Complex Assembly and Selective Gene Activation
BioProject PRJNA186697
SRA SRP017968

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Supplementary file Size Download File type/resource
GSE43540_RAW.tar 2.6 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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