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Series GSE43526 Query DataSets for GSE43526
Status Public on Aug 30, 2013
Title Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors [Seq]
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Topoisomerases are necessary for the expression of neurodevelopmental genes, and are mutated in some patients with autism spectrum disorder (ASD). We have studied the effects of inhibitors of Topoisomerase 1 (Top1) and Topoisomerase 2 (Top2) enzymes on mouse cortical neurons. We find that topoisomerases selectively inhibit long genes (>100kb), with little effect on all other gene expression. Using ChIPseq against RNA Polymerase II (Pol2) we show that the Top1 inhibitor topotecan blocks transcriptional elongation of long genes specifically. Many of the genes inhibited by topotecan are candidate ASD genes, leading us to propose that topoisomerase inhibition might contribute to ASD pathology.
Overall design [Mouse] 5 biological replicates of transcriptome sequencing (RNAseq) from topotecan treated neurons and vehicle treated controls; Pol2 ChIPseq of topotecan and vehicle treated neurons
[Human] Transcriptome sequencing (RNAseq) from topotecan treated neurons and vehicle treated control.
Contributor(s) King IF, Yandava C
Citation(s) 23995680
Submission date Jan 16, 2013
Last update date May 15, 2019
Contact name Chandri Yandava
Organization name University of North Carolina at Chapel Hill
Department Carolina Institute for Developmental Disabilities
Street address 101 Renee Lynne Court
City Carrboro
State/province NC
ZIP/Postal code 27510
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (14)
GSM1064933 Vehicle_RNAseq_rep1 [mouse]
GSM1064934 Vehicle_RNAseq_rep2 [mouse]
GSM1064935 Vehicle_RNAseq_rep3 [mouse]
This SubSeries is part of SuperSeries:
GSE43900 Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors
BioProject PRJNA186671
SRA SRP017966

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Supplementary file Size Download File type/resource
GSE43526_RAW.tar 1.9 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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