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Series GSE43476 Query DataSets for GSE43476
Status Public on Jan 15, 2013
Title Genomic Reprogramming Errors Do Not Accumulate with Serial Recloning in the Mouse
Organism Mus musculus
Experiment type Expression profiling by array
Summary To date, the success rate of serial animal cloning has decreased with increasing iterations and recloning has failed in all species after a few generations. This has suggested that recloning might be impossible because of the accumulation of lethal genetic or epigenetic abnormalities. Here, we carried out repeated recloning in the mouse using our somatic cell nuclear transfer method combined with a histone deacetylase inhibitor. The success rate of recloning did not decrease even after 25 iterations and more than 500 viable offspring have been obtained from a single original donor. Although the genomic reprogrammability of somatic cell nuclei did not improve, reprogramming errors or clone-specific abnormalities did not accumulate with recloning. Our results provide the first evidence that iterative recloning is possible, suggesting that animals might be able to be recloned indefinitely.
 
Overall design Brain and liver samples from 4 somatic cell clone (CC), 4 somatic cell clones of 20 generations (G20) and 4 normal controls (ICSI) neonate.
 
Contributor(s) Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T
Citation(s) 23472871
Submission date Jan 14, 2013
Last update date Jan 12, 2017
Contact name Takashi Kohda
E-mail(s) tkohda.epgn@mri.tmd.ac.jp
Organization name Tokyo Medical and Dental University
Department Medical Research Institute
Lab Epigenetics
Street address 1-5-45 Yushima, Bunko-ku
City Tokyo
ZIP/Postal code 113-8510
Country Japan
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (24)
GSM1063412 Brain_NC_1
GSM1063413 Brain_NC_2
GSM1063414 Brain_NC_3
Relations
BioProject PRJNA186527

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43476_RAW.tar 189.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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