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Series GSE43262 Query DataSets for GSE43262
Status Public on Jan 01, 2015
Title Ascorbic acid enhances Tet-mediated 5-methylcytosine oxidation and promotes DNA demethylation in mammals.
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary DNA demethylation occurs in multiple cellular processes but its regulation is poorly understood. Here we report that a vital nutrient factor ascorbic acid (AA), or vitamin C (Vc), induces DNA demethylation in mammalian cells. The levels of active 5mC oxidation products, 5-formylcytosine and 5-carboxylcytosine, increase by more than one magnitude of order in mouse ES cells when cultured in the presence of AA. In cells deleted of Tet1 and Tet2 dioxygenase genes, AA does not facilitate 5mC oxidation. The AA effect is however restored when Tet2 was re-expressed in the mutant cells. The enhancing effect of AA on 5mC oxidation and DNA demethylation was also observed in a mouse model deficient in AA synthesis. Our results thus reveal a role of AA in the regulation of DNA modification, which might mediate a plethora of functions of AA.
Overall design Examination of 5hmC levels with or without AA in mouse ES cells
Contributor(s) Yang Y, Xu G, Wang H
Citation(s) 23768208
Submission date Jan 03, 2013
Last update date May 15, 2019
Contact name Zechen Chong
Organization name Beijing Institute of Genomics
Street address NO. 7 Beitucheng West Road
City Beijing
ZIP/Postal code 100029
Country China
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1059599 5hmC_V
GSM1059600 5hmC_C
BioProject PRJNA185172
SRA SRP017761

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43262_RAW.tar 2.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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