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Series GSE43169 Query DataSets for GSE43169
Status Public on Sep 30, 2013
Title Gene expression profiling of mature dendritic cells from mice s. c. treated with GM-CSF-gene transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Irradiated granulocyte macrophage-colony stimulating factor (GM-CSF)-transduced autologous tumor cells induce substantial antitumor immunity through the maturation and migration of dendritic cells (DCs). However, little is known about the key molecules involved in GM-CSF-sensitized DCs (GM-DCs) in tumor draining lymph nodes (TDLNs). We initially confirmed that mice subcutaneously injected with poorly immunogenic syngeneic Lewis lung carcinoma (LLC) cells transduced with Sendai virus encoding GM-CSF (LLC/SeV/GM) significantly rejected the tumor growth. Using microarray expression profiling, we obtained a large number of gene expression data files from GM-DCs and control DCs in TDLNs, and subjected them to network-based cluster analysis and unexpectedly unraveled the expression levels of type I IFNs-related genes specifically expressed in plasmacytoid DCs (pDC) were robustly up-regulated in GM-DCs. In vivo depletion assay showed that pDC-depleted mice treated with subcutaneous LLC/SeV/GM cells abrogated the antitumor effects observed in control mice. Moreover combination use of imiquimod for TLR7 triggering on pDC with irradiated LLC/SeV/GM cells induced a significant therapeutic antitumor effect with marked induction of CD9+ pDC with antitumor phenotype, whereas other control mice groups had only minimal to-modest antitumor responses, implicating that this combined vaccine strategy using imiquimod could be promising for improvement of GM-CSF-induced antitumor immunity.
Overall design Mouse GM-CSF induced gene expression in mature dendritic cells in tumor draining lymph nodes from C57/BL6N female mouse was measured at 2 days after s.c. tumor challenge with GM-CSF gene-transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP).
Contributor(s) Narusawa M, Inoue H, Sakamoto C, Inoue T, Watanabe A, Miyamoto S, Miura Y, Hijikata Y, Inoue M, Takayama K, Okazaki T, Hasegawa M, Nakanishi Y, Tani K
Citation(s) 24830413
Submission date Dec 27, 2012
Last update date Nov 01, 2017
Contact name kenzaburo tani
Organization name Kyushu University
Department Medical Institute of Bioregulation
Lab Department of Molecular Genetics
Street address 3-1-1 Maidashi
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (3)
GSM1057800 CD86 positive DCs LLC rep1
GSM1057801 CD86 positive DCs LLC/SeV/GFP rep1
GSM1057802 CD86 positive DCs LLC/SeV/GM rep1
BioProject PRJNA184817

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43169_RAW.tar 26.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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