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Series GSE43169 Query DataSets for GSE43169
Status Public on Sep 30, 2013
Title Gene expression profiling of mature dendritic cells from mice s. c. treated with GM-CSF-gene transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Irradiated granulocyte macrophage-colony stimulating factor (GM-CSF)-transduced autologous tumor cells induce substantial antitumor immunity through the maturation and migration of dendritic cells (DCs). However, little is known about the key molecules involved in GM-CSF-sensitized DCs (GM-DCs) in tumor draining lymph nodes (TDLNs). We initially confirmed that mice subcutaneously injected with poorly immunogenic syngeneic Lewis lung carcinoma (LLC) cells transduced with Sendai virus encoding GM-CSF (LLC/SeV/GM) significantly rejected the tumor growth. Using microarray expression profiling, we obtained a large number of gene expression data files from GM-DCs and control DCs in TDLNs, and subjected them to network-based cluster analysis and unexpectedly unraveled the expression levels of type I IFNs-related genes specifically expressed in plasmacytoid DCs (pDC) were robustly up-regulated in GM-DCs. In vivo depletion assay showed that pDC-depleted mice treated with subcutaneous LLC/SeV/GM cells abrogated the antitumor effects observed in control mice. Moreover combination use of imiquimod for TLR7 triggering on pDC with irradiated LLC/SeV/GM cells induced a significant therapeutic antitumor effect with marked induction of CD9+ pDC with antitumor phenotype, whereas other control mice groups had only minimal to-modest antitumor responses, implicating that this combined vaccine strategy using imiquimod could be promising for improvement of GM-CSF-induced antitumor immunity.
 
Overall design Mouse GM-CSF induced gene expression in mature dendritic cells in tumor draining lymph nodes from C57/BL6N female mouse was measured at 2 days after s.c. tumor challenge with GM-CSF gene-transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP).
 
Contributor(s) Narusawa M, Inoue H, Sakamoto C, Inoue T, Watanabe A, Miyamoto S, Miura Y, Hijikata Y, Inoue M, Takayama K, Okazaki T, Hasegawa M, Nakanishi Y, Tani K
Citation(s) 24830413
Submission date Dec 27, 2012
Last update date Nov 01, 2017
Contact name kenzaburo tani
E-mail(s) taniken@bioreg.kyushu-u.ac.jp
Organization name Kyushu University
Department Medical Institute of Bioregulation
Lab Department of Molecular Genetics
Street address 3-1-1 Maidashi
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (3)
GSM1057800 CD86 positive DCs LLC rep1
GSM1057801 CD86 positive DCs LLC/SeV/GFP rep1
GSM1057802 CD86 positive DCs LLC/SeV/GM rep1
Relations
BioProject PRJNA184817

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43169_RAW.tar 26.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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