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Series GSE43153 Query DataSets for GSE43153
Status Public on Oct 10, 2013
Title Antiviral RNA interference in mammalian cells
Organism Mus musculus
Experiment type Other
Non-coding RNA profiling by high throughput sequencing
Summary Plants and invertebrates protect themselves from viruses through RNA interference (RNAi), yet it remains unknown whether this defense mechanism exists in mammals. Antiviral RNAi involves the processing of viral long double-stranded (ds) RNA molecules into small interfering RNAs (siRNAs) by the ribonuclease (RNAse) III Dicer. These siRNAs are incorporated into effector complex(es) containing members of the Argonaute (Ago) protein family and guide silencing of complementary target viral RNAs. Here, we detect the accumulation of phased Dicer-dependent virus-derived siRNA (viRNAs) and demonstrate their loading into Ago2 after infection of mouse embryonic stem (ES) cells with Encephalomyocarditis virus (EMCV). We further show that the production of these viRNAs is drastically reduced, yet not completely abolished, if ES cells are first induced to differentiate before infection. Finally, we reveal that the mammalian virus Nodamura virus (NoV) encodes for a protein that counteracts such antiviral RNAi in ES cells supporting the existence of an effective RNAi-based immunity in mammals.
Overall design Infection of wild-type or mutant mouse ES cells and analysis of small RNAs from total extracts or immunoprecipitated components of the RNAi pathway
Contributor(s) Maillard PV, Ciaudo C, Marchais A, Li Y, Jay F, Ding S, Voinnet O
Citation(s) 24115438
Submission date Dec 26, 2012
Last update date May 15, 2019
Contact name Antonin Marchais
Phone +41 44 632 64 08
Organization name ETHZ
Department Department of Biology
Lab Group Prof. Olivier Voinnet
Street address Universitatstrasse 2
City Zurich
ZIP/Postal code 8092
Country Switzerland
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM1057530 ES_NI
GSM1057531 ES_EMCV 3hrs
GSM1057532 ES_EMCV 6hrs
BioProject PRJNA184730
SRA SRP017691

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Supplementary file Size Download File type/resource
GSE43153_RAW.tar 670.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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