GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE42999 Query DataSets for GSE42999
Status Public on Dec 18, 2014
Title β3 agonist administration before reperfusion reduces infarct size and improves long term cardiac function in small and large animal models of myocardial infarction
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA sequencing and subsequent bioinformatics analyses were performed at early reoxygenation stages in HL-1 cardiomyocytes treated or not with BRL37344
Methods: HL-1 cardiomyocytes were subjected to Hypoxia/Reoxygenation (6h/1h) with/without a β3AR agonist (BRL37344 5µmol/L). mRNA profiles were generated by deep sequencing, in triplicate, using Illumina GAIIx.The sequence reads that passed quality filters were quantified using BWA aligned reads using RSEM. qRT–PCR validation was performed using SYBR Green assay.
Results: After 6h of hypoxia followed by 1h reoxygenation, 866 genes were differentially expressed upon β3AR stimulation by BRL37344. Among these, 177 were at least 2-fold up or downregulated.
Conclusions: Our results show that Hsp70 plays a key role in the cardioprotection afforded by β3AR agonism in cardiomyocytes during the early window of H/R.
Overall design mRNA profiles from cardiomyocyte subjected to H/R (6h/1h) with/without a β3AR agonist were generated by deep sequencing, in triplicate, using Illumina GAIIx.
Contributor(s) Sanz-Rosa D, Garcia-Prieto J, Ibanez B
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 18, 2012
Last update date May 15, 2019
Contact name David Sanz-Rosa
Organization name CNIC
Department DEAIC
Lab IExC
Street address 3, Melchor Fernandez Almagro
City Madrid
ZIP/Postal code 28029
Country Spain
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (6)
GSM1054685 HL-1 + Vehicle rep 1
GSM1054686 HL-1 + Vehicle rep 2
GSM1054687 HL-1 + Vehicle rep 3
BioProject PRJNA184170
SRA SRP017600

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42999_RAW.tar 3.7 Mb (http)(custom) TAR (of TXT)
GSE42999_RSEM_normalized_counts.txt.gz 385.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap