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Series GSE42801 Query DataSets for GSE42801
Status Public on Aug 07, 2013
Title SAM domain polymerization links subnuclear clustering of PRC1 to silencing (Ring1B ChIP-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb group (PcG) proteins mediate heritable but reversible silencing of developmental regulator genes by modifying their chromatin configuration. Accumulating evidence documents a role for PcG proteins in regulating higher order chromatin structures likely by their clustering, however, underlying mechanisms and its impact on transcriptional regulation remain obscure. In this study, we found that subnuclear clustering of PRC1 at canonical PcG target genes depended on head-to-tail polymerization property of SAM domain of Phc2 and likely Phc1. We show that Phc2-SAM polymerization limits the dynamic nature of PRC1, thereby promotes stable association of PRC1 with PcG target genes and contributes to their robust silencing. Our findings suggest a novel model by which SAM polymerization of Phc2 modulates the structural organization of PcG complexes to enable robust yet reversible PcG-mediated repression during development.
Overall design Examination of Ring1B in wild type and the Phc2 mutant cells
Contributor(s) Sharif J, Endo TA
Citation(s) 24091011
Submission date Dec 07, 2012
Last update date May 15, 2019
Contact name Takaho A. Endo
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1050218 MEF_WT_Ring1B
GSM1050219 MEF_L307R_Ring1B
This SubSeries is part of SuperSeries:
GSE38154 SAM domain polymerization links subnuclear clustering of PRC1 to silencing detected using expression data and ChIP experiments
BioProject PRJNA183216
SRA SRP017468

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Supplementary file Size Download File type/resource
GSE42801_RAW.tar 4.4 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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