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Series GSE42684 Query DataSets for GSE42684
Status Public on Apr 08, 2013
Title High natural gene expression variation in the reef-building coral Acropora millepora: Potential for acclimative and adaptive plasticity
Organism Acropora millepora
Experiment type Expression profiling by array
Summary This experiment assessed the natural gene expression variation present between colonies of the Indo-Pacific reef-building coral Acropora millepora, and additionally explored whether gene expression differed between two different intron haplotypes according to intron 4-500 in a carbonic anhydrase homolog. This study found no correspondence between host genotype and transcriptional state, but found significant intercolony variation, detecting 488 representing unique genes or 17% of the total genes analyzed. Such transcriptomic variation could be the basis upon which natural selection can act. Underlying variation could potentially allow reef corals to respond to different environments. Whether this source of variation and the genetic responses of corals and its symbionts will allow coral reefs to cope to the rapid pace of global change remains unknown.
Overall design A. millepora colonies were brought to a common garden in the reef lagoon, i.e. under the same environmental conditions. This common garden combined with acclimatization removes environmental effects on the physiology of the coral colonies. For the comparison of the two intron haplotypes, we applied a multiple dye-swap microarray design for the two groups of coral colonies (N=3 per group) defined based on the two genotypes resolved with the use of intron 4-500 (Fig. 1). To also examine the intra-haplotype variation we added a loop design nested to the above multiple dye-swap design, where three samples per colony were included. Colonies 1, 2, and 3 are of intron 4-500 haplotype 1; colonies 4, 5, and 6 are haplotype 2.
Contributor(s) Bellantuono AJ, Granados-Cifuentes C
Citation(s) 23565725
Submission date Dec 03, 2012
Last update date Dec 04, 2017
Contact name Anthony John Bellantuono
Organization name Florida International University
Department Biological Sciences
Lab Rodriguez-Lanetty
Street address 11200 SW 8th
City Miami
State/province FL
ZIP/Postal code 33199
Country USA
Platforms (1)
GPL6941 Australian National University Acropora millepora 18k array version 1
Samples (22)
GSM1047911 Colony 1 (cy5) - Colony 4 (cy3)
GSM1047912 Colony 1-1 (cy5) - Colony 2-2 (cy3)
GSM1047913 Colony 1-2 (cy5) - Colony 2-3 (cy3)
BioProject PRJNA182731

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42684_RAW.tar 33.3 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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