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Series GSE42376 Query DataSets for GSE42376
Status Public on Apr 04, 2013
Title The coregulator, repressor of Estrogen Receptor Activity (REA), is a crucial regulator of the timing and magnitude of uterine decidualization.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Our findings establish a key role for the coregulator, Repressor of Estrogen receptor Activity (REA), in controlling the timing and magnitude of decidualization in human endometrial stromal cells in vitro and in the mouse uterus in vivo, and suggest that REA functions to synchronize uterine differentiation with concurrent embryo development, which is essential for optimal implantation and fertility.
The findings highlight that REA physiologically restrains endometrial stromal cell decidualization, controlling the timing and magnitude of decidualization to enable proper synchronization of uterine differentiation with concurrent embryo development that is essential for implantation and optimal fertility.
Overall design Human endometrial stromal cells (hESCs) were isolated from biopsies taken from the early proliferative stage endometrium of regularly cycling women on no hormonal medications. Cells were cultured in DMEM/F-12 mediumcontaining 5% charcoal stripped fetal bovine serum. To induce in vitro decidualization, hESCs were treated with a hormone cocktail containing 10 nM estradiol (E2), 1 μM progesterone (P4) and 0.5 uM 8-bromo-cAMP for up to 10 days, and media were changed every 48 h. For siRNA experiments, hESCs were transfected with REA siRNA or GL3 luciferase control siRNA following the Silent-Fect kit protocol. After 48 h of transfection, hESCs were exposed to the hormone cocktail for 24 hours or 96 hours of differentiation.
key words; siRNA knock-down, hormone cocktail treatment
Contributor(s) Zhao Y, Katzenellenbogen B
Citation(s) 23392257
Submission date Nov 19, 2012
Last update date Dec 06, 2018
Contact name Yuechao Zhao
Organization name University of Illinois
Street address 407 S. Goodwin Ave
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM1038475 1 = B7_siGL3_24h
GSM1038476 2 = B7_KO_24h
GSM1038477 3 = N16_siGL3_24h
BioProject PRJNA181203

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Supplementary file Size Download File type/resource
GSE42376_RAW.tar 15.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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