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Series GSE42298 Query DataSets for GSE42298
Status Public on Mar 01, 2013
Title Rif1 Prevents Resection of DNA Breaks and Promotes Immunoglobulin Class Switching
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary DNA double-strand breaks (DSBs) represent a threat to the genome because they can lead to loss of genetic information and chromosome rearrangements. The DNA repair protein p53 binding protein 1 (53BP1) protects the genome by limiting nucleolytic processing of DSBs by a mechanism that requires its phosphorylation, but whether it does so directly is not known. Here we identify Rapl-interacting factor 1 (Rif1) as an Ataxia-Telangiectasia Mutated (ATM) phosphorylation-dependent interactor of 53BP1, and show that absence of Rif1 results in 5’-3’ DNA end resection in mice. Consistent with enhanced DNA resection, Rif1 deficiency impairs DNA repair in the G1 and S phases of the cell cycle, interferes with class switch recombination (CSR) in B lymphocytes, and leads to accumulation of chromosome DSBs.
Overall design Study of Rif1 DNA-end protection activity against resection via analysis of single-stranded DNA binding protein RPA and Rad51 accumulation at sites of AID-induced DNA damage by ChIP-seq. All samples shown in Fig. 4 are included (controls and test samples, 7 samples in total).
Contributor(s) Di Virgilio M, Callen E, Yamane A, Zhang W, Jankovic M, Gitlin AD, Feldhahn N, Resch W, Oliveira TY, Chait BT, Nussenzweig A, Casellas R, Robbiani DF, Nussenzweig MC
Citation(s) 23306439
Submission date Nov 14, 2012
Last update date May 15, 2019
Contact name Thiago Y Oliveira
Organization name The Rockefeller University
Department Immunology, Virology and Microbiology
Lab Laboratory of Molecular Immunology
Street address 1230 YORK AVE
State/province New York
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (7)
GSM1037389 RPA_ChIPSeq_53BP1_panel4A
GSM1037390 RPA_ChIPSeq_Rif1_panel4A
GSM1037391 RPA_ChIPSeq_Cd19_panel4A
BioProject PRJNA181019
SRA SRP017184

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Supplementary file Size Download File type/resource
GSE42298_RAW.tar 456.3 Mb (http)(custom) TAR (of WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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