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Series GSE4225 Query DataSets for GSE4225
Status Public on Jul 28, 2006
Title Sox4 knockdown in ACC3 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Sox4 is upregulated in adenoid cystic carcinoma (ACC). The ACC3 cell line is the most widely available cell culture model system of this tumor type. RNAi mediated knockdown of Sox4 in ACC3 cells induces apoptosis. Since Sox4 is a transcription factor, it is hypothesized that this apoptotic phenotype is due to changes in transcription from genes under Sox4 control.This experiment was designed to find candidate genes whose expression levels change after Sox4 knockdown that could contribute to the pro-apoptotic phenotype.
Keywords: gene knockdown
Overall design The ACC3 cell line was subjected to plasmid vector-based shRNA knockdown of the Sox4 gene, with a control group consisting of cells transfected with a plasmid vector expressing shRNA for the bacterial lacZ gene. Both groups were performed in triplicate.The shRNA sequences were expressed from the plasmid pENTR/U6 (Life Technologies Inc). Sox4 sequence: 5’-GACGACCTGCTCGACCTGAcgaaTCAGGTCGAGCAGGTCGTC-3’. RNA was harvested 48 hours after transfection.Labeled cRNA probe was hybridized to Affymetrix U133a probes.
Contributor(s) Moskaluk CA, Pramoonjago P, Baras AS
Citation(s) 16636670
Submission date Feb 09, 2006
Last update date Aug 10, 2018
Contact name Christopher A. Moskaluk
Phone 434 982-4408
Fax 434 243-6757
Organization name Christopher Moskaluk
Department Pathology
Street address P.O. Box 800214, OMS Rm. 3890, Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (6)
GSM96347 ACC 3 cell line lacZ-RNAi replicate 1
GSM96348 ACC 3 cell line lacZ-RNAi replicate 2
GSM96349 ACC 3 cell line lacZ-RNAi replicate 3
BioProject PRJNA94871

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